Mouse NF- kappa- B inhibitor alpha, Nfkbia ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NF-kappa-B inhibitor alpha,I-kappa-B-alpha(IkB-alpha,IkappaBalpha),RL/IF-1,Nfkbia,IkappaBalpha,NF-kappa-B inhibitor alpha; IKBA; MAD-3; NFKBI; I-kappa-B-alpha; ikB-alpha; major histocompatibility complex enhancer-binding protein MAD3; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Search name
Mouse NF-kappa-B inhibitor alpha ELISA KIT ,Mouse I-kappa-B-alpha ELISA KIT ,Mouse IkB-alpha ELISA KIT ,Mouse IkappaBalpha ELISA KIT ,Mouse RL/IF-1 ELISA KIT ,Mouse Nfkbia ELISA KIT ,Mouse IkappaBalpha ELISA KIT ,Mouse NF-kappa-B inhibitor alpha ELISA KIT ,Mouse IKBA ELISA KIT ,Mouse MAD-3 ELISA KIT ,Mouse NFKBI ELISA KIT ,Mouse I-kappa-B-alpha ELISA KIT ,Mouse ikB-alpha ELISA KIT ,Mouse major histocompatibility complex enhancer-binding protein MAD3 ELISA KIT ,Mouse nuclear factor of kappa light chain gene enhancer in B-cells ELISA KIT ,Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse NF-kappa-B inhibitor alpha concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NF-kappa-B inhibitor alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NF-kappa-B inhibitor alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NF-kappa-B inhibitor alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NF-kappa-B inhibitor alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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