Mouse Uridine phosphorylase 1,UPP1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
UPP1,UDRPASE; UPASE; UPP; UPase 1; urdPase 1; uridine phosphorylase 1, uridine phosphorylase
Search name
Mouse UPP1 ELISA KIT ,MouseUDRPASE ELISA KIT ,MouseUPASE ELISA KIT ,MouseUPP ELISA KIT ,MouseUPase 1 ELISA KIT ,MouseurdPase 1 ELISA KIT ,Mouseuridine phosphorylase 1 ELISA KIT ,Mouse uridine phosphorylase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination ofmouseUPase 1concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to UPase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forUPase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain UPase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UPase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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