Mouse Cluster of differentiation 5, CD5 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
T-cell surface glycoprotein CD5,Lymphocyte antigen T1/Leu-1, CD5,CD5 molecule; T-cell surface glycoprotein CD5; CD5 antigen
(p56-62); LEU1; lymphocyte antigen T1/Leu-1
Search name
Mouse Cluster of differentiation 5 ELISA Kit, Mouse CD5 ELISA Kit, Mouse T-cell surface glycoprotein CD5 ELISA Kit, Mouse
Lymphocyte antigen T1/Leu-1 ELISA Kit, Mouse Lymphocyte antigen T1ELISA Kit, Mouse Leu-1 ELISA Kit, Mouse CD5
molecule ELISA Kit, Mouse CD5 antigen ELISA Kit, Mouse p56-62 ELISA Kit, Mouse LEU1 ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse t-cell surface glycoprotein cd5,T-cell surface glycoprotein
CD5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-cell surface glycoprotein CD5. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for T-cell
surface glycoprotein CD5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then
a TMB substrate solution is added to each well. Only those wells that contain T-cell surface glycoprotein CD5, biotin-conjugated antibody
and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric
acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of T-cell
surface glycoprotein CD5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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