Mixed lineage kinase domain-like protein, Mlkl ELISA KIT/ Mouse Mixed lineage kinase domain-like protein, Mlkl ELISA试剂盒

Mouse Mixed lineage kinase domain- like protein, MLKL ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

MLKL,hMLKL; mixed lineage kinase domain-like

 

Search name

Mouse MLKL ELISA KIT ,Mouse hMLKL ELISA KIT,Mouse mixed lineage kinase domain-like ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse hMLKL concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to hMLKL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forhMLKL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain hMLKL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of hMLKL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

 

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