Mouse Probable D- lactate dehydrogenase mitochondrial, LDHD ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Probable D-lactate dehydrogenase, mitochondrial,DLD,Lactate dehydrogenase D,LDHD,DLD; D-lactate dehydrogenase; lactate dehydrogenase D
Search name
Mouse Probable D-lactate dehydrogenase mitochondrial ELISA KIT ,Mouse DLD ELISA KIT ,Mouse Lactate dehydrogenase D ELISA KIT ,Mouse LDHD ELISA KIT ,Mouse DLD ELISA KIT ,Mouse D-lactate dehydrogenase ELISA KIT ,Mouse lactate dehydrogenase D ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse probable d-lactate dehydrogenase, mitochondrial,DLD concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DLD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DLD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DLD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DLD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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