Mouse Phosphoglycerate mutase 2, PGAM2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Phosphoglycerate mutase 2,Phosphoglycerate mutase isozyme M,PGAM-M,BPG-dependent PGAM 2,Muscle-specific phosphoglycerate mutase, PGAM2,GSD10; PGAM-M; PGAMM; BPG-dependent PGAM 2; muscle-specific phosphoglycerate mutase; phosphoglycerate mutase 2; phosphoglycerate mutase isozyme M; phosphoglycerate mutase 2
Search name
Mouse Phosphoglycerate mutase 2 ELISA KIT ,Mouse Phosphoglycerate mutase isozyme M ELISA KIT ,Mouse PGAM-M ELISA KIT ,Mouse BPG-dependent PGAM 2 ELISA KIT ,Mouse Muscle-specific phosphoglycerate mutase ELISA KIT ,Mouse PGAM2 ELISA KIT ,Mouse GSD10 ELISA KIT ,Mouse PGAM-M ELISA KIT ,Mouse PGAMM ELISA KIT ,Mouse BPG-dependent PGAM 2 ELISA KIT ,Mouse muscle-specific phosphoglycerate mutase ELISA KIT ,Mouse phosphoglycerate mutase 2 ELISA KIT ,Mouse phosphoglycerate mutase isozyme M ELISA KIT ,Mouse phosphoglycerate mutase 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse phosphoglycerate mutase 2,PGAM2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGAM2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PGAM2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PGAM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGAM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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