Mouse Chitinase- 3- like protein 1, CHI3L1 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Chitinase-3-like protein 1,YKL-40,39 kDa synovial protein,Cartilage glycoprotein 39,CGP-39,GP-39,hCGP-39, CHI3L1,ASRT7; CGP-39; GP-39; GP39; HC-gp39; HCGP-3P; YKL-40; YKL40; YYL-40; hCGP-39; 39 kDa synovial protein; cartilage glycoprotein 39; chitinase-3-like protein 1; chitinase 3-like 1 cartilage glycoprotein-39
Search name
Mouse Chitinase-3-like protein 1 ELISA KIT ,Mouse YKL-40 ELISA KIT ,Mouse 39 kDa synovial protein ELISA KIT ,Mouse Cartilage glycoprotein 39 ELISA KIT ,Mouse CGP-39 ELISA KIT ,Mouse GP-39 ELISA KIT ,Mouse hCGP-39 ELISA KIT ,Mouse CHI3L1 ELISA KIT ,Mouse ASRT7 ELISA KIT ,Mouse CGP-39 ELISA KIT ,Mouse GP-39 ELISA KIT ,Mouse GP39 ELISA KIT ,Mouse HC-gp39 ELISA KIT ,Mouse HCGP-3P ELISA KIT ,Mouse YKL-40 ELISA KIT ,Mouse YKL40 ELISA KIT ,Mouse YYL-40 ELISA KIT ,Mouse hCGP-39 ELISA KIT ,Mouse 39 kDa synovial protein ELISA KIT ,Mouse cartilage glycoprotein 39 ELISA KIT ,Mouse chitinase-3-like protein 1 ELISA KIT ,Mouse chitinase 3-like 1 cartilage glycoprotein-39 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse chitinase-3-like protein 1,Chitinase-3-like protein 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chitinase-3-like protein 1, During the reaction, Chitinase-3-like protein 1 in the sample or standard competes with a fixed amount of biotin-labeled Chitinase-3-like protein 1 for sites on a pre-coated Monoclonal antibody specific to Chitinase-3-like protein 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Chitinase-3-like protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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