Mouse Mitogen- activated protein kinase kinase kinase 5, MAP3K5 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MAP3K5,ASK1,MAPKKK5; MEKK5; ASK-1; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; apoptosis signal regulating kinase 1; apoptosis signal-regulating kinase 1; mitogen-activated protein kinase kinase kinase 5
Search name
Mouse MAP3K5 ELISA KIT ,Mouse ASK1 ELISA KIT ,Mouse MAPKKK5 ELISA KIT ,Mouse MEKK5 ELISA KIT ,Mouse ASK-1 ELISA KIT ,Mouse MAP/ERK kinase kinase 5 ELISA KIT ,Mouse MAPK/ERK kinase kinase 5 ELISA KIT ,Mouse MEK kinase 5 ELISA KIT ,Mouse MEKK 5 ELISA KIT ,Mouse apoptosis signal regulating kinase 1 ELISA KIT ,Mouse apoptosis signal-regulating kinase 1 ELISA KIT ,Mouse mitogen-activated protein kinase kinase kinase 5 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Mitogen- activated protein kinase kinase kinase 5, MAP3K5 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAP3K5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MAP3K5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MAP3K5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MAP3K5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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