Mouse Osteopontin, OPN ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Osteopontin,Uropontin,Urinary stone protein,Secreted phosphoprotein 1(SPP-1),Bone sialoprotein 1,Nephropontin, osteopontin,secreted
phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); osteopontin/immunoglobulin alpha 1 heavy chain
constant region fusion protein; BNSP; BSPI; ETA-1; OPN; SPP1/CALPHA1 fusion; early T-lymphocyte activation 1; nephropontin; urinary
stone protein; uropontin; secreted phosphoprotein 1
SearCh name
Mouse Osteopontin ELISA Kit, Mouse OPN ELISA Kit, Mouse Uropontin ELISA Kit, Mouse Urinary stone protein ELISA Kit,
Mouse SeCreted phosphoprotein 1 ELISA Kit, Mouse SPP-1 ELISA Kit, Mouse Bone sialoprotein 1 ELISA Kit, Mouse
Nephropontin ELISA Kit, Mouse bone sialoprotein I ELISA Kit, Mouse early T-lymphoCyte aCtivation 1 ELISA Kit, Mouse
immunoglobulin alpha 1 heavy Chain Constant region fusion protein ELISA Kit, Mouse BNSP ELISA Kit, Mouse BSPI ELISA Kit,
Mouse ETA-1 ELISA Kit, Mouse SPP1 ELISA Kit, Mouse CALPHA1 fusion ELISA Kit, Mouse early T-lymphoCyte aCtivation 1
ELISA Kit, Mouse nephropontin; urinary stone protein ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Osteopontin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteopontin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Osteopontin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Osteopontin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Osteopontin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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