Mouse Glycogen phosphorylase, liver form, Pygl ELISA KIT Product Name:Mouse Glycogen phosphorylase, liver form, Pygl ELISA KIT
Packing:96T
Catalog No.:ELI-02893m
Gene Name:Mouse Pygl
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Pygl
Alternative Name:Mouse Glycogen phosphorylase, liver form, Pygl
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Glycogen phosphorylase, liver form, Pygl ELISA KIT allows for the in vitro quantitative determination of Mouse Pygl concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Glycogen phosphorylase, liver form, Pygl ELISA KIT has been pre-coated with an Mouse Glycogen phosphorylase, liver form, Pygl antibody specific to Mouse Pygl .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pygl and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pygl , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pygl in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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