Mouse Glucose-6-phosphate 1-dehydrogenase X, G6PD ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Glucose-6-phosphate 1-dehydrogenase,G6PD,G6PD1; glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate dehydrogenase,glucose 6 phosphate dehydrogenase,G6pdx, Glucose-6-phosphate 1-dehydrogenase X
Search name
Mouse Glucose-6-phosphate 1-dehydrogenase ELISA KIT ,Mouse G6PD ELISA KIT ,Mouse G6PD1 ELISA KIT ,Mouse glucose-6-phosphate 1-dehydrogenase ELISA KIT ,Mouse glucose-6-phosphate dehydrogenase ELISA KIT ,Mouse glucose 6 phosphate dehydrogenase ELISA KIT ,Mouse G6pdx ELISA KIT ,Mouse Glucose-6-phosphate 1-dehydrogenase X ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse glucose-6-phosphate 1-dehydrogenase x,G6PD concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to G6PD. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for G6PD and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain G6PD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of G6PD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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