Creatine Kinase ELISA kit/ Mouse Creatine Kinase ELISA试剂盒

Mouse Creatine Kinase ELISA kit

Product Name:Mouse Creatine Kinase ELISA kit
Packing:96T

Catalog No.:ELA-E2030m

Gene Name:Mouse CK

Detect Range:0.156-10ng/ml

Sensitivity:0.094ng/ml

Target Protein Name:Mouse CK

Alternative Name:Mouse Creatine Kinase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Creatine Kinase ELISA kit allows for the in vitro quantitative determination of Mouse CK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Creatine Kinase ELISA kit has been pre-coated with an Mouse Creatine Kinase antibody specific to Mouse CK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse CK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse CK , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse CK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

 

 

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