Product Name:Mouse Arachidonate 5- Lipoxygenase ELISA KIT
Packing:96T
Catalog No.:ELA-E1355m
Gene Name:Mouse 5-LOX(ALOX5
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse 5-LOX(ALOX5
Alternative Name:Mouse Arachidonate 5-Lipoxygenase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Arachidonate 5- Lipoxygenase ELISA KIT allows for the in vitro quantitative determination of Mouse 5-LOX(ALOX5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Arachidonate 5- Lipoxygenase ELISA KIT has been pre-coated with an Mouse Arachidonate 5-Lipoxygenase antibody specific to Mouse 5-LOX(ALOX5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse 5-LOX(ALOX5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse 5-LOX(ALOX5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse 5-LOX(ALOX5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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