Mouse anti- extractable nuclear antigen antibody ELISA Kit Product Name:Mouse anti- extractable nuclear antigen antibody ELISA Kit
Packing:96T
Catalog No.:ELA-E1124m
Gene Name:Mouse ENA-Ab
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse ENA-Ab
Alternative Name:Mouse anti-extractable nuclear antigen antibody
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse anti- extractable nuclear antigen antibody ELISA Kit allows for the in vitro quantitative determination of Mouse ENA-Ab concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse anti- extractable nuclear antigen antibody ELISA Kit has been pre-coated with an Mouse anti-extractable nuclear antigen antibody antibody specific to Mouse ENA-Ab .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse ENA-Ab and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse ENA-Ab , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse ENA-Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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