Product Name:Mouse cholesterol 7- alpha- hydroxylase ELISA Kit
Packing:96T
Catalog No.:ELA-E1053m
Gene Name:Mouse CYP7A1
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse CYP7A1
Alternative Name:Mouse cholesterol 7-alpha-hydroxylase
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse cholesterol 7- alpha- hydroxylase ELISA Kit allows for the in vitro quantitative determination of Mouse CYP7A1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse cholesterol 7- alpha- hydroxylase ELISA Kit has been pre-coated with an Mouse cholesterol 7-alpha-hydroxylase antibody specific to Mouse CYP7A1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse CYP7A1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse CYP7A1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse CYP7A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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