Mouse Chorionic Gonadotrophin Beta, Beta- HCG ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CGB,CGB3; CGB5; CGB7; CGB8; hCGB; CG-beta; chorionic gonadotrophin chain beta; chorionic gonadotropin beta 3 subunit; chorionic gonadotropin beta chain; chorionic gonadotropin beta subunit; chorionic gonadotropin beta polypeptide, Choriogonadotropin subunit beta, Chorionic Gonadotropin β,β-CG
Search name
Mouse CGB ELISA KIT ,Mouse CGB3 ELISA KIT ,Mouse CGB5 ELISA KIT ,Mouse CGB7 ELISA KIT ,Mouse CGB8 ELISA KIT ,Mouse hCGB ELISA KIT ,Mouse CG-beta ELISA KIT ,Mouse chorionic gonadotrophin chain beta ELISA KIT ,Mouse chorionic gonadotropin beta 3 subunit ELISA KIT ,Mouse chorionic gonadotropin beta chain ELISA KIT ,Mouse chorionic gonadotropin beta subunit ELISA KIT ,Mouse chorionic gonadotropin beta polypeptide ELISA KIT ,Mouse Choriogonadotropin subunit beta ELISA KIT ,Mouse Chorionic Gonadotropin β ELISA KIT ,Mouse β-CG ELISA KIT ,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse choriogonadotropin subunit beta,CG-beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CG-beta. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CG-beta and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CG-beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CG-beta in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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