Mouse Heme oxygenase 1, Hmox1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Heme oxygenase 1,P32 protein, HMOX1,HO-1,HMOX1D,HSP32,bK286B10,heat shock protein, 32-kD,heme oxygenase 1,heme oxygenase decycling 1
Search name
Mouse Heme oxygenase 1 ELISA KIT ,Mouse P32 protein ELISA KIT ,Mouse HMOX1 ELISA KIT ,Mouse HO-1 ELISA KIT ,Mouse HMOX1D ELISA KIT ,Mouse HSP32 ELISA KIT ,Mouse bK286B10 ELISA KIT ,Mouse heat shock protein ELISA KIT ,Mouse 32-kD ELISA KIT ,Mouse heme oxygenase 1 ELISA KIT ,Mouse heme oxygenase decycling 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse heme oxygenase 1,HO-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HO-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HO-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HO-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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