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pESC- HIS/ pESC- HIS质粒/ pESC- HIS Plasmid

产品信息
  • pESC-HIS

    Catalog No. PVT11233
    Packing 2ug

     

    pESC-HIS Information

    Function yeast expression plasmid

    Resistance Amp

    Promoter: GAL1, GAL10 promoter

    Plasmid size: 6706bp

    Screening marker: DH5 alpha

    Clone strains: 37 DEG C, aerobic, LB

     

    pESC-HIS Description

    The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae.  Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter.  These vectors also feature an extensive polylinker sequence and the ability to generate end-specific RNA transcripts from T3 and T7 promoters.  Each of the pESC vectors contains one of four different yeast-selectable markers (HIS3, TRP1, LEU2, or URA3) in the same vector backbone.
               The pESC vectors contain DNA sequences coding for epitope peptides that can be specifically recognized by monoclonal antibodies.   A sequence for the FLAG® epitope (DYKDDDDK) is located in the multiple cloning site (MCS) downstream of the GAL10 promoter; a sequence for the c-Myc epitope (EQKLISEEDL) is located in the MCS downstream of the GAL1 promoter. You can insert your gene of interest in front of the epitope sequence to generate C-terminal tagging or after the epitope sequence for N-terminal tagging. These tags allow the protein of interest to be studied without generating a specific antibody to that protein. The epitope-tagged fusion proteins can be studied in transformed cells using well-characterized antibodies.
               • Express two different genes simultaneously in S. cerevisiae 
               • Proteins tagged with unique epitope tags 
               • Fast and easy immunoprecipitations
     
    pESC-HIS Sequence
    LOCUS       Exported                6706 bp ds-DNA     circular SYN 13-APR-2017
    DEFINITION  synthetic circular DNA
    ACCESSION   .
    VERSION     .
    KEYWORDS    pESC-HIS
    SOURCE      synthetic DNA construct
      ORGANISM  synthetic DNA construct
    REFERENCE   1  (bases 1 to 6706)
      AUTHORS   .
      TITLE     Direct Submission
           
    FEATURES             Location/Qualifiers
         source          1..6706
                         /organism="synthetic DNA construct"
                         /mol_type="other DNA"
         promoter        317..503
                         /gene="S. cerevisiae HIS3"
                         /note="HIS3 promoter"
         CDS             504..1163
                         /codon_start=1
                         /gene="S. cerevisiae HIS3"
                         /product="imidazoleglycerol-phosphate dehydratase, required
                         for histidine biosynthesis"
                         /note="HIS3"
                         /note="yeast auxotrophic marker"
                         /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                         QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                         KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                         ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
         rep_origin      complement(1423..1878)
                         /direction=LEFT
                         /note="f1 ori"
                         /note="f1 bacteriophage origin of replication; arrow 
                         indicates direction of (+) strand synthesis"
         terminator      1961..2126
                         /gene="S. cerevisiae ADH1"
                         /note="ADH1 terminator"
                         /note="transcription terminator for the S. cerevisiae 
                         alcohol dehydrogenase 1 (ADH1) gene"
         CDS             complement(2274..2297)
                         /codon_start=1
                         /product="FLAG(R) epitope tag, followed by an enterokinase 
                         cleavage site"
                         /note="FLAG"
                         /translation="DYKDDDDK"
         promoter        2344..3008
                         /gene="GAL1 and GAL10 genes of S. cerevisiae"
                         /note="GAL1,10 promoter"
                         /note="divergent inducible promoter, regulated by Gal4"
         protein_bind    2560..2677
                         /bound_moiety="Gal4"
                         /note="UAS"
                         /note="upstream activating sequence mediating 
                         Gal4-dependent induction"
         promoter        3019..3037
                         /note="T7 promoter"
                         /note="promoter for bacteriophage T7 RNA polymerase"
         CDS             3055..3084
                         /codon_start=1
                         /product="Myc (human c-Myc oncogene) epitope tag"
                         /note="Myc"
                         /translation="EQKLISEEDL"
         terminator      3114..3303
                         /gene="S. cerevisiae CYC1"
                         /note="CYC1 terminator"
                         /note="transcription terminator for CYC1"
         rep_origin      complement(3546..4134)
                         /direction=LEFT
                         /note="ori"
                         /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                         replication"
         CDS             complement(4305..5165)
                         /codon_start=1
                         /gene="bla"
                         /product="beta-lactamase"
                         /note="AmpR"
                         /note="confers resistance to ampicillin, carbenicillin, and
                         related antibiotics"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                         ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                         PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                         EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                         LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                         LIKHW"
         promoter        complement(5166..5270)
                         /gene="bla"
                         /note="AmpR promoter"
         rep_origin      5297..6639
                         /note="2u ori"
                         /note="yeast 2u plasmid origin of replication"
         protein_bind    complement(6260..6307)
                         /bound_moiety="FLP recombinase from the Saccharomyces 
                         cerevisiae 2u plasmid"
                         /note="FRT"
                         /note="FLP-mediated recombination occurs in the 8-bp core 
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