pLKO.1-EGFP-Puro
Catalog No. PVT11103
Packing 2ug
Function /
Resistance Amp
Screen Puro/EGFP
Strain Stbl3
Culture temperature 37degrees centigrade
Replicon pUC
Copy high
Promoter U6
Induction --
Forward primer U6
Reverse primer
Prokaryotic resistance: ampicillin Amp
Screening marker: puromycin Puro, green fluorescent protein EGFP
Clone strain: Escherichia coli Stbl3
Culture conditions: 37 C
Expression host: lactation cells
PLKO.1-EGFP-Puro is a lactation cell lentivirus interfering with RNA plasmid.
pLKO.1-EGFP-Puro Sequence
LOCUS Exported 8301 bp ds-DNA circular SYN 14-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..8301
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 7..507
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
CDS 529..1128
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
LTR 1256..1489
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 1561..1682
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 1722..1857
/label=SV40 ori
/note="SV40 origin of replication"
rep_origin 2064..2519
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2545..2649
/gene="bla"
/label=AmpR promoter
CDS 2650..3510
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3681..4269
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 4738..4964
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 4965..5145
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 5192..5317
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5810..6043
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 6570..6810
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
enhancer 6832..7135
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 7136..7339
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 7368..7377
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 7374..8093
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 8132..8249
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
温馨提示：不可用于临床ZL。
