pLenti CMV V5-LUC Blast (w567-1)
Catalog No. PVT11097
Packing 2ug
pLenti CMV V5-LUC Blast (w567-1) Informaiton
Promoter: CMV promoter
Replicator: Ori, F1 ori, SV40 ori
Terminator: WPRE
Plasmid classification: virus series plasmid; lentiviral plasmid; virus expression plasmid.
Plasmid size: 9463bp
Plasmid tagging: N-V5
Prokaryotic resistance: ampicillin Amp
Screening marker: magnaporin Blast
Clone strain: Escherichia coli Stbl3
Culture conditions: 37 C, aerobic, LB
Expression host: mammalian cells such as 293T
pLenti CMV V5-LUC Blast (w567-1) Description
3rd gen lentiviral V5-Luciferase expression vector, CMV promoter, Blast.
pLenti CMV V5-LUC Blast (w567-1) Reference
A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells .
pLenti CMV V5-LUC Blast (w567-1) Sequence
LOCUS Exported 9463 bp ds-DNA circular SYN 17-鍏湀-2017
KEYWORDS pLenti CMV V5-LUC Blast (w567-1)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9463)
FEATURES Location/Qualifiers
source 1..9463
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 27..131
/gene="bla"
/note="AmpR promoter"
CDS 132..992
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1163..1751
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
LTR 2448..2628
/note="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 2672..2797
/note="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 3290..3523
/note="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 4012..4129
/note="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 4152..4455
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 4456..4659
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
protein_bind 4764..4788
/gene="mutant version of attB"
/bound_moiety="BP Clonase(TM)"
/note="attB1"
/note="recombination site for the Gateway(R) BP reaction"
CDS 4800..4841
/codon_start=1
/product="epitope tag from simian virus 5"
/note="V5"
/translation="GKPIPNPLLGLDST"
CDS 4917..6569
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/EC_number="
"
/note="luciferase"
/note="enhanced luc+ version of the luciferase gene"
/protein_id="
"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
protein_bind complement(6576..6600)
/gene="mutant version of attB"
/bound_moiety="BP Clonase(TM)"
/note="attB2"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 6631..7219
/note="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
promoter 7275..7605
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 7456..7591
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 7653..7700
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 7719..8117
/codon_start=1
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温馨提示：不可用于临床ZL。
