pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Catalog No. PVT11052
Packing 2ug
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Information
Promoter: PGK
Replicon: pUC ori
Plasmid classification: virus series, reverse virus cloning cloning vector
Plasmid size: 8500bp
Prokaryotic resistance: Amp
Screening markers: Puro
Cloned strain: Stbl3
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, instantaneous expression
5'sequencing primers: primers designed according to sequence
3'sequencing primers: primers designed according to sequence
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Description
PMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE is Expression Retroviral, Mammalian, Cre/Lox carrier, its background carrier is MSCV, insert SEQ is inserted. loxp-dsRed-loxp-MCS-eGFP.
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Sequence
LOCUS Exported 8500 bp ds-DNA circular SYN 06-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 13
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8500)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, September 6, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..8500
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 580..921
/note="MESV Psi"
/note="packaging signal of murine embryonic stem cell
virus"
CDS 988..1404
/note="gag (truncated)"
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
protein_bind 1438..1471
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS 1506..2183
/codon_start=1
/product="noncytotoxic tetrameric variant of DsRed
fluorescent protein (Strack et al., 2008)"
/note="DsRed-Express2"
/note="mammalian codon-optimized"
/translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
LFQ"
protein_bind 2208..2241
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS 2298..3017
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
promoter 3027..3526
/note="PGK promoter"
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 3547..4146
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="PuroR"
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
misc_feature 4216..4804
/note="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(4687..4698)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/note="Factor Xa site"
/translation="IEGR"
LTR 4861..5376
/note="3' LTR"
/note="3' long terminal repeat from murine embryonic stem
cell virus"
primer_bind complement(5545..5561)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 5569..5585
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5593..5623)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 5638..5659
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5947..6535)
/direction=LEFT
温馨提示：不可用于临床ZL。
