pSilencer 4.1-CMV puro-Stuffer
Catalog No. PVT10871
Packing 2ug
Function Mammal Editing plasmids
Resistance Amp
Screen Puro
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon pUC
Copy high
Promoter
Induction
Forward primer
Reverse primer
pSilencer 4.1-CMV puro-Stuffer Description
PSilencer 4.1-CMV puro-Stuffer is a mammalian cell interfering RNA expression plasmid.
pSilencer 4.1-CMV puro-Stuffer Sequence
LOCUS Exported 6679 bp ds-DNA circular SYN 19-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..6679
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(1586..1789)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer 1790..2093
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
rep_origin complement(2685..3273)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3444..4304)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4305..4409)
/gene="bla"
/label=AmpR promoter
polyA_signal 4497..4578
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(4808..5407)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
promoter complement(5504..5820)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5518..5653
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 6235..6293
/label=CMV pA signal
misc_feature 6299..1564
/label=Stuffer
ORIGIN
1 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
61 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
121 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
181 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
241 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
301 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
361 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
421 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
481 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
541 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
601 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
661 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
721 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
781 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
841 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
901 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
961 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
1021 tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg
1081 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
1141 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
1201 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
1261 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc
1321 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
1381 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
1441 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
1501 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
1561 ataaggatcc acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc
1621 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
1681 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
1741 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
1801 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
1861 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
1921 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
1981 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
2041 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
2101 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctaagatctg
2161 gtaccttgaa ttcatgcttc tcctcccttt agtgagggta attctctctc tctccctata
2221 gtgagtcgta ttaattcctt ctcttctata gtgtcaccta aatcgttgca attcgtaatc
2281 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
2341 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
2401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
2461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
2521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
2581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
2641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
2701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
2761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
2821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
2881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
2941 cacgaacccc ccgttcagcc <
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