MG15-luc (mut p53 binding sites)
Catalog No. PVT10824
Packing 2ug
Function Mammal reporter plasmid
Resistance Amp
Screen Luc
Strain DH5α
Culture temperature LB/37鈩?br /> Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
MG15-luc (mut p53 binding sites) Sequence
LOCUS Exported 6013 bp ds-DNA circular SYN 27-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6013)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 27, 2017
FEATURES Location/Qualifiers
source 1..6013
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 9..178
/label=mut p53 binding sites
CDS 480..2132
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
intron 2263..2328
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 2458..2478
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 2903..3037
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3580..4168)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4339..5199)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5200..5304)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5330..5785)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5950..5968
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 agcttgatcc ttaatggact ttaatggcct tatggacttt aatggcctta atggacttta
61 atggccttaa tggactttaa tggccttaat ggactttaat ggccttaatg gactttaatg
121 gccttaatgg actttaatgg ccttaatgga ctttaatggc cttaatggac tttaatggcc
181 atcgaattcg atctgttttt tttagtatta agcagaggcc gggggcccct ggcctccgct
241 tactctggag aaaaagaaga gaggcattcc agaggcaact tgtcaaaaca ggactggcgc
301 cttggaggcg ctgtggggcc acccaaattg atataattaa gccccaaccg cctcttcccg
361 cctcatttca gcctcaccac cctcgacaag cttatcgata ccgtcggggg atccactagt
421 tctagagcgg ccgccaccgc ggtggagctc gagcttggca ttccggtact gttggtaaaa
481 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatcctcta gaggatggaa
541 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
601 cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg
661 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
721 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
781 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgaacattt
841 cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
901 aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat taccagggat
961 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
1021 ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaat tcctctggat
1081 ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc agattctcgc
1141 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
1201 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
1261 gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt caggattaca
1321 aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa agcactctga
1381 ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca cctctttcga
1441 aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga caaggatatg
1501 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
1561 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
1621 cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt atgtccggtt
1681 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
1741 gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc ttgaagtctt
1801 taattaaata caaaggatat caggtggccc ccgctgaatt ggaatcgata ttgttacaac
1861 accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc ggtgaacttc
1921 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
1981 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
2041 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
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