pRL-TK-intronless
Catalog No. PVT10807
Packing 2ug
Function Mammal reporter plasmid
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
pRL-TK-intronless Description
PRL-TK-intronless is a fluorescein reporter plasmid for breast-feeding sea kidney.
pRL-TK-intronless Sequence
LOCUS Exported 3811 bp ds-DNA circular SYN 03-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..3811
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 8..759
/label=HSV TK promoter
/note="herpes simplex virus thymidine kinase promoter"
promoter 772..790
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 800..1735
/codon_start=1
/product="luciferase from the anthozoan coelenterate
Renilla reniformis (sea pansy)"
/label=Rluc
/translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
polyA_signal 1766..1887
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 2020..2124
/gene="bla"
/label=AmpR promoter
CDS 2125..2985
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3156..3744
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg
361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat
481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat
541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttagtac ttaatacgac
781 tcactatagg ctagccacca tgacttcgaa agtttatgat ccagaacaaa ggaaacggat
841 gataactggt ccgcagtggt gggccagatg taaacaaatg aatgttcttg attcatttat
901 taattattat gattcagaaa aacatgcaga aaatgctgtt atttttttac atggtaacgc
961 ggcctcttct tatttatggc gacatgttgt gccacatatt gagccagtag cgcggtgtat
1021 tataccagac cttattggta tgggcaaatc aggcaaatct ggtaatggtt cttataggtt
1081 acttgatcat tacaaatatc ttactgcatg gtttgaactt cttaatttac caaagaagat
1141 catttttgtc ggccatgatt ggggtgcttg tttggcattt cattatagct atgagcatca
1201 agataagatc aaagcaatag ttcacgctga aagtgtagta gatgtgattg aatcatggga
1261 tgaatggcct gatattgaag aagatattgc gttgatcaaa tctgaagaag gagaaaaaat
1321 ggttttggag aataacttct tcgtggaaac catgttgcca tcaaaaatca tgagaaagtt
1381 agaaccagaa gaatttgcag catatcttga accattcaaa gagaaaggtg aagttcgtcg
1441 tccaacatta tcatggcctc gtgaaatccc gttagtaaaa ggtggtaaac ctgacgttgt
1501 acaaattgtt aggaattata atgcttatct acgtgcaagt gatgatttac caaaaatgtt
1561 tattgaatcg gacccaggat tcttttccaa tgctattgtt gaaggtgcca agaagtttcc
1621 taatactgaa tttgtcaaag taaaaggtct tcatttttcg caagaagatg cacctgatga
1681 aatgggaaaa tatatcaaat cgttcgttga gcgagttctc aaaaatgaac aataattcta
1741 gagcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
1801 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
1861 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
1921 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
1981 atcgataagg atccaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
2041 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
2101 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
2161 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
2221 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
2281 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
2341 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
2401 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
2461 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
2521 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
2581 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
2641 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
2701 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
2761 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
2821 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
2881 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
2941 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
3001 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
3061 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
3121 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
3181 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
3241 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
3301 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
3361 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
3421 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
3481 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
3541 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
温馨提示：不可用于临床ZL。
