pBARGPE1-mCherry Search name
pBARGPE1-mCherry,Plasmid pBARGPE1-mCherry,pBARGPE1-mCherry vector
pBARGPE1-mCherry Information
Use:Filamentous fungal plasmid
Promoter: gpdA, TrpC
Replicon: pUC ori
Terminator: trpC
Plasmid classification: other host shuttle vectors; filamentous fungi expressing plasmids.
Plasmid size: 6229bp
Plasmid label: mono red fluorescent protein mCherry
Prokaryotic resistance: ampicillin Ampicillin
Screening markers: LP Bar
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: filamentous fungi
Induction mode: no induction
5'sequencing primers: Based on full sequence design
3'sequencing primers: Based on full sequence design
pBARGPE1-mCherry Description
PBARGPE1-mCherry is a filamentous fungus - Escherichia coli shuttle expression vector, which can use gpdA promoter to activate the expression of foreign gene, and can be transformed into filamentous fungi to be screened by gpdA.
pBARGPE1-mCherry Sequence
LOCUS Exported 6229 bp ds-DNA circular SYN 30-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBARGPE1-mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6229)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..6229
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 28..258
/note="trpC terminator"
protein_bind complement(652..685)
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
protein_bind complement(763..796)
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS complement(1188..1739)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/note="BlpR"
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
misc_feature complement(1740..2125)
/note="TrpC Promotor"
promoter complement(2216..2317)
/gene="S. cerevisiae TRP1"
/note="TRP1 promoter"
promoter 2423..2527
/gene="bla"
/note="AmpR promoter"
CDS 2528..3388
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3559..4147
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 4333..4473
/note="bom"
/note="basis of mobility region from pBR322"
misc_feature 4688..5372
/note="gpdA Promoter"
primer_bind complement(5422..5438)
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
CDS 5474..6184
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/note="mCherry"
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(6196..6212)
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gccccgtccg gtcctgcccg tcaccgagat ccacttaacg ttactgaaat catcaaacag
61 cttgacgaat ctggatataa gatcgttggt gtcgatgtca gctccggagt tgagacaaat
121 ggtgttcagg atctcgataa gatacgttca tttgtccaag cagcaaagag tgccttctag
181 tgatttaata gctccatgtc aacaagaata aaacgcgttt cgggtttacc tcttccagat
241 acagctcatc tgcaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
301 caattataca tttaatacgc gatagaaaac aaaatatagc gcgtgtacgc atgtaacatt
361 atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcaagctt
421 tgaagaaaaa tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg
481 gaagacattt gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg
541 tgggaaattg gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga
601 taacagcaat acctgatcga ccaattccga tcatattcaa taacccttaa tataacttcg
661 tataatgtat gctatacgaa gttattaggt ctgaagagga gtttacgtcc agccaagcta
721 gcttggctgc aggtcgagcg gccgcgatcc ggaaccctta atataacttc gtataatgta
781 tgctatacga agttattagg tccctcgacc ggccgcggcg gttgtttgca agaccgagaa
841 aaggctagca agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag
温馨提示：不可用于临床ZL。
