Search name
pSV-Beta-Gal,Plasmid pSV-Beta-Gal,pSV-Beta-Gal vector,pSV-Beta-Gal mappSV-Beta-Gal Information
Promoter: SV40
Replicon: SV40 ori
Terminator: SV40 poly (A) signal
Plasmid classification: mammalian cell carrier; protein overexpression plasmid.
Plasmid size: 6820bp
Plasmid label: LacZ
Prokaryotic resistance: ampicillin Amp
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: mammalian cells
Culture conditions: 37 centigrade, aerobic, LB
Induction mode: no induction, instantaneous expression
5'sequencing primers: Based on sequence design
3'sequencing primers: Based on sequence design
pSV-Beta-Gal Description
pSV-Beta-Gal Sequence
LOCUS Exported 6820 bp ds-DNA circular SYN 28-鍏湀-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pSV-beta-gal
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6820)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2016-09-18 from SnapGene Viewer 2.8.1
FEATURES Location/Qualifiers
source 1..6820
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 56..413
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 264..399
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 710..3757
/codon_start=1
/gene="lacZ"
/product="beta-galactosidase"
/note="beta-galactosidase"
/translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
SWSPSVSAEFQLSAGRYHYQLVWCQK"
polyA_signal 4021..4155
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(4189..4205)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 4679..4783
/gene="bla"
/note="AmpR promoter"
CDS 4784..5644
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5815..6403
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 6691..6712
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6727..6757
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 6765..6781
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6789..6805
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
61 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
121 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
241 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
301 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
361 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
421 ggatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa
481 acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat
541 ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc
601 cgtaagccgt ggcggtctgg taccggtggg tgaagaccag aaacagcacc tcgaactgag
