pCambia 1391Xb Alternative Name
pCambia 1391Xb,pCambia 1391Xb plasmid, pCambia 1391Xb vector, pCambia 1391Xb map
pCambia 1391Xb Information
Plasmid type: plant expression vector
High copy / low copy: low copy
Promoter: -
Cloning methods: polyclonal sites, restrictive endonucleases
Carrier size: 10647 BP
5'sequencing primers and sequences: --
3'sequencing primers and sequences: --
Carrier label: GusA
Carrier resistance: kanana and Hygromycin
Screening markers: HPTII
Note: -
Stability: stable expression
Composition type: non group molding
Virus / non virus: non virus
pCambia 1391Xb Sequence
LOCUS pCAMBIA1391Xb 10647 bp ds-DNA circular SYN
DEFINITION Agrobacterium binary vector with hygromycin- and
kanamycin-resistance and GUS genes plus the pUC9 MCS. For other
reading frames, use pCAMBIA1391Xa or pCAMBIA1391Xc.
ACCESSION AF234310
VERSION .
KEYWORDS pCAMBIA1391Xb
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
COMMENT This file is created by Vector NTI
COMMENT The GenBank record was corrected by inserting a G at position 4541.
FEATURES Location/Qualifiers
source 1..10647
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
CDS 20..1825
/codon_start=1
/gene="uidA?(or gusA)?from E. coli"
/product="beta-glucuronidase"
/note="GUS"
CDS 1832..1849
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 1881..2133
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 2155..2179
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS 3479..4108
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/note="pVS1 StaA"
CDS 4537..5610
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/note="pVS1 RepA"
/note="
"
/protein_id="
"
misc_feature 6214..6354
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(6540..7128)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7215..8009)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/protein_id="
"
misc_feature 8434..8458
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 8536..8710
/note="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(8750..9775)
/codon_start=1
/product="hygromycin B phosphotransferase"
/note="HygR"
/note="confers resistance to hygromycin"
promoter complement(9843..10520)
/note="CaMV 35S promoter (enhanced)"
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
misc_feature 10609..10644
/note="MCS"
/note="multiple cloning site from pUC9"
ORIGIN
1 cgctgtagat ctgactagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact
61 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg
121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt
181 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat
241 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg
301 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga
361 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa
421 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa
481 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat
541 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca
601 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga
661 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca
721 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac
781 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc
841 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg
901 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca
961 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga
1021 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt
1081 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta
1141 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct
1201 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga
1261 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact
1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac
1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag
1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa
1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc
1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg
1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg
1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga
1801 aaaaccgcag cagggaggca aacaagctag ccaccaccac caccaccacg tgtgaattgg
1861 tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
1921 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
1981 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
2041 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
2101 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag tgtttgacag
2161 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa
2221 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
2281 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
2341 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
2401 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
2461 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
2521 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
2581 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
2641 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
2701 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
2761 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
2821 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
2881 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
2941 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
3001 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
3061 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
3121 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
3181 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
3241 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
3301 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
3361 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
3421 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
3481 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
3541 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
3601 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
3661 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
3721 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
3781 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
3841 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
3901 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
3961 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
4021 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
4081 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
4141 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
4201 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
4261 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
4321 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
4381 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
4441 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg
4501 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc
4561 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
4621 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
4681 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
4741 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
4801 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
4861 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
4921 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
4981 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
5041 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
5101 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
5161 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
5221 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
5281 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
5341 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
5401 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
5461 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
5521 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
5581 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
5641 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac
5701 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
5761 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
5821 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
5881 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
5941 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
6001 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
6061 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
6121 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
6181 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
6241 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
6301 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
6361 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
6421 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
6481 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
6541 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
6601 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
6661 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
6721 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
6781 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
6841 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
6901 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
6961 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
7021 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
7081 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
7141 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
7201 atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa
7261 tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc
7321 tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc
7381 ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg
7441 tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc
7501 tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga
7561 tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga
7621 caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc
7681 ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc
7741 atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc
7801 tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta
7861 taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc
7921 ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc
7981 aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt
8041 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
8101 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
8161 acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc
8221 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
8281 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
8341 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
8401 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
8461 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact gaattaacgc
8521 cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga attagaaatt
8581 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
8641 acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact cacacattat
8701 tatggagaaa ctcgagcttg tcgatcgaca gatccggtcg gcatctactc tatttctttg
8761 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
8821 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
8881 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
8941 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg
9001 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
9061 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
9121 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
9181 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
9241 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
9301 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
9361 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
9421 tagcctccgc gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
9481 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt
9541 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
9601 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
9661 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
9721 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc
9781 attgcccccc gggatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
9841 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg tcttgcgaag
9901 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
9961 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
10021 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
10081 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
10141 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
10201 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
10261 ccatgttatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
10321 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
10381 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
10441 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
10501 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
10561 tggagtagac gagagtgtcg tgctccacca tgttgggccc ggcgcgccaa gcttggctgc
10621 aggtcgacgg atccccggga attccag
//
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