基本信息
| 启动子: | OpIE-1，EM7 |
| 复制子: | pUC ori |
| 终止子: | SV40 poly(A) signal |
| 质粒分类: | 广宿主系列，昆虫细胞载体 |
| 质粒大小: | 3336bp |
| 原核抗性: | ZeO |
| 克隆菌株: | DH5α |
| 培养条件: | 37℃，有氧 LB |
| 表达宿主: | 昆虫细胞 |
| 诱导方式: | 无须诱导，瞬时表达 |
| 5'测序引物: | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
| 3'测序引物: | 根据序列设计引物 |
| 备注: | 大肠中无荧光，哺乳细胞中有绿色荧光 |
质粒简介
pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistanceprotein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lineswith Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
质粒图谱
质粒序列
LOCUS Exported 3336 bp ds-DNA circular SYN 07-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 12
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3336)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, September 7, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..3336
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 5..552
/gene="immediate-early gene 2 from the Orgyia pseudotsugata
multicapsid nucleopolyhedrovirus"
/note="OpIE-2 promoter"
/note="strong constitutive baculovirus promoter for insect
cell expression"
CDS 663..704
/codon_start=1
/product="epitope tag from simian virus 5"
/note="V5 tag"
/translation="GKPIPNPLLGLDST"
CDS 714..731
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
polyA_signal 749..878
/gene="immediate-early gene 2 from the Orgyia pseudotsugata
multicapsid nucleopolyhedrovirus"
/note="OpIE-2 poly(A) signal"
/note="baculovirus polyadenylation signal"
rep_origin complement(1006..1594)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 1669..1960
/gene="immediate-early gene 1 from the Orgyia pseudotsugata
multicapsid nucleopolyhedrovirus"
/note="OpIE-1 promoter"
/note="moderate constitutive baculovirus promoter for
insect cell expression"
promoter 1986..2033
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 2766..3143
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MDAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWG
REFALRDPAGNCVHFVAEEQD"
polyA_signal 3207..5
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
ORIGIN
1 ggatcatgat gataaacaat gtatggtgct aatgttgctt caacaacaat tctgttgaac
61 tgtgttttca tgtttgccaa caagcacctt tatactcggt ggcctcccca ccaccaactt
121 ttttgcactg caaaaaaaca cgcttttgca cgcgggccca tacatagtac aaactctacg
181 tttcgtagac tattttacat aaatagtcta caccgttgta tacgctccaa atacactacc
241 acacattgaa cctttttgca gtgcaaaaaa gtacgtgtcg gcagtcacgt aggccggcct
301 tatcgggtcg cgtcctgtca cgtacgaatc acattatcgg accggacgag tgttgtctta
361 tcgtgacagg acgccagctt cctgtgttgc taaccgcagc cggacgcaac tccttatcgg
421 aacaggacgc gcctccatat cagccgcgcg ttatctcatg cgcgtgaccg gacacgaggc
481 gcccgtcccg cttatcgcgc ctataaatac agcccgcaac gatctggtaa acacagttga
541 acagcatctg ttcgaattta aagcttggta ccgagctcgg atccactagt ccagtgtggt
601 ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg
661 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
721 accatcacca ttgagtttat ctgactaaat cttagtttgt attgtcatgt tttaatacaa
781 tatgttatgt ttaaatatgt ttttaataaa ttttataaaa taatttcaac ttttattgta
841 acaacattgt ccatttacac actcctttca agcgcgtggg atcgatgctc actcaaaggc
901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
1021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
1081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
1141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
1201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
1261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
1321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
1381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
1441 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
1501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
1561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
1621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc gaaacacgca
1681 cggcgcgcgc acgcagctta gcacaaacgc gtcgttgcac gcgcccaccg ctaaccgcag
1741 gccaatcggt cggccggcct catatccgct caccagccgc gtcctatcgg gcgcggcttc
1801 cgcgcccatt ttgaataaat aaacgataac gccgttggtg gcgtgaggca tgtaaaaggt
1861 tacatcatta tcttgttcgc catccggttg gtataaatag acgttcatgt tggtttttgt
1921 ttcagttgca agttggctgc ggcgcgcgca gcacctttgc cgggatctgc cgggctgcag
1981 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
2041 ggaactaaac catggcggta gaaaaaatgg ctagcaaagg agaagaactt ttcactggag
2101 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
2161 gagagggtga aggtgatgct acatacggaa agcttaccct taaatttatt tgcactactg
2221 gaaaactacc tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct
2281 tttcccgtta tccggatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag
2341 gttatgtaca ggaacgcact atatctttca aagatgacgg gaactacaag acgcgtgctg
2401 aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaaaggt attgatttta
2461 aagaagatgg aaacattctc ggacacaaac tcgagtacaa ctataactca cacaatgtat
2521 acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgtcacaaca
2581 ttgaagatgg atccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
2641 gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgccctt tcgaaagatc
2701 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac
2761 atggcatgga tgccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
2821 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
2881 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
2941 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
3001 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
3061 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
3121 tcgtggccga ggagcaggac tgaccgacgc cgaccaacac cgccggtccg acgcggcccg
3181 acgggtccga ggggggtcga cctcgaaact tgtttattgc agcttataat ggttacaaat
3241 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
3301 gtttgtccaa actcatcaat gtatcttatc atgtct
温馨提示：不可用于临床ZL。
