基本信息
| 启动子: | OpIE-2 promoter |
| 复制子: | ori |
| 终止子: | OpIE-2 poly(A) signal |
| 质粒分类: | 其他宿主载体；昆虫细胞质粒 |
| 质粒大小: | 3688bp |
| 质粒标签: | N-Dsred2，C-V5，C-6×His |
| 原核抗性: | 博来霉素Zeo |
| 筛选标记: | 博来霉素Zeo |
| 克隆菌株: | 大肠杆菌DH5α |
| 培养条件: | LB培养基，37℃ |
质粒简介
pIZT-DsRed2-V5-6×His昆虫表达质粒质粒图谱
质粒序列
LOCUS Exported 3688 bp ds-DNA circular SYN 28-JAN-2019
DEFINITION synthetic circular DNA
KEYWORDS pIZT-DsRed2-V5-His
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3688)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3688
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 5..552
/gene="immediate-early gene 2 from the Orgyia pseudotsugata
multicapsid nucleopolyhedrovirus"
/note="OpIE-2 promoter"
/note="strong constitutive baculovirus promoter for insect
cell expression"
CDS 567..1241
/codon_start=1
/product="improved tetrameric variant of DsRed fluorescent
protein"
/note="DsRed2"
/note="mammalian codon-optimized"
/translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
LFL"
CDS 1475..1516
/codon_start=1
/product="epitope tag from simian virus 5"
/note="V5 tag"
/translation="GKPIPNPLLGLDST"
CDS 1526..1543
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
polyA_signal 1561..1690
/gene="immediate-early gene 2 from the Orgyia pseudotsugata
multicapsid nucleopolyhedrovirus"
/note="OpIE-2 poly(A) signal"
/note="baculovirus polyadenylation signal"
rep_origin complement(1818..2406)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 2478..3025
/gene="immediate-early gene 2 from the Orgyia pseudotsugata
multicapsid nucleopolyhedrovirus"
/note="OpIE-2 promoter"
/note="strong constitutive baculovirus promoter for insect
cell expression"
promoter 3054..3101
/note="EM7 promoter"
/note="synthetic bacterial promoter "
promoter 3093..3111
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 3115..3124
/regulatory_class="other"
/note="Kozak sequence"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3121..3495
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="Zeo"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 3559..3680
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
ORIGIN
1 ggatcatgat gataaacaat gtatggtgct aatgttgctt caacaacaat tctgttgaac
61 tgtgttttca tgtttgccaa caagcacctt tatactcggt ggcctcccca ccaccaactt
121 ttttgcactg caaaaaaaca cgcttttgca cgcgggccca tacatagtac aaactctacg
181 tttcgtagac tattttacat aaatagtcta caccgttgta tacgctccaa atacactacc
241 acacattgaa cctttttgca gtgcaaaaaa gtacgtgtcg gcagtcacgt aggccggcct
301 tatcgggtcg cgtcctgtca cgtacgaatc acattatcgg accggacgag tgttgtctta
361 tcgtgacagg acgccagctt cctgtgttgc taaccgcagc cggacgcaac tccttatcgg
421 aacaggacgc gcctccatat cagccgcgcg ttatctcatg cgcgtgaccg gacacgaggc
481 gcccgtcccg cttatcgcgc ctataaatac agcccgcaac gatctggtaa acacagttga
541 acagcatctg ttcgaattta aagcttatgg cctcctccga gaacgtcatc accgagttca
601 tgcgcttcaa ggtgcgcatg gagggcaccg tgaacggcca cgagttcgag atcgagggcg
661 agggcgaggg ccgcccctac gagggccaca acaccgtgaa gctgaaggtg accaagggcg
721 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt
781 acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca
841 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggc gaccgtgacc caggactcct
901 ccctgcagga cggctgcttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg
961 acggccccgt gatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc
1021 cccgcgacgg cgtgctgaag ggcgagaccc acaaggccct gaagctgaag gacggcggcc
1081 actacctggt ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct
1141 actactacgt ggacgccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
1201 agcagtacga gcgcaccgag ggccgccacc acctgttcct gggtaccgag ctcggatccc
1261 ccccgaagaa ctgtacccac ttaggcggct gtaatagcga ctgtctgacc cgtagcgaga
1321 ttcaagcact gtttagagaa gcgataaaca cactgaaaca cacaatgaat actgaagacg
1381 agtgcgctca catgttggat atagtgtcat tcgaaagaat caaggagtac attcgcgccg
1441 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
1501 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt atctgactaa
1561 atcttagttt gtattgtcat gttttaatac aatatgttat gtttaaatat gtttttaata
1621 aattttataa aataatttca acttttattg taacaacatt gtccatttac acactccttt
1681 caagcgcgtg ggatcgatgc tcactcaaag gcggtaatac ggttatccac agaatcaggg
1741 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
1801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
1861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
1921 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
1981 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
2041 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
2101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
2161 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
2221 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
2281 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
2341 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
2401 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
2461 cgttaaggga ttttggtcat gatgataaac aatgtatggt gctaatgttg cttcaacaac
2521 aattctgttg aactgtgttt tcatgtttgc caacaagcac ctttatactc ggtggcctcc
2581 ccaccaccaa cttttttgca ctgcaaaaaa acacgctttt gcacgcgggc ccatacatag
2641 tacaaactct acgtttcgta gactatttta cataaatagt ctacaccgtt gtatacgctc
2701 caaatacact accacacatt gaaccttttt gcagtgcaaa aaagtacgtg tcggcagtca
2761 cgtaggccgg ccttatcggg tcgcgtcctg tcacgtacga atcacattat cggaccggac
2821 gagtgttgtc ttatcgtgac aggacgccag cttcctgtgt tgctaaccgc agccggacgc
2881 aactccttat cggaacagga cgcgcctcca tatcagccgc gcgttatctc atgcgcgtga
2941 ccggacacga ggcgcccgtc ccgcttatcg cgcctataaa tacagcccgc aacgatctgg
3001 taaacacagt tgaacagcat ctgttcgaat taattcggat ctctgcagca cgtgttgaca
3061 attaatcatc ggcatagtat atcggcatag tataatacga ctcactatag gagggccacc
3121 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
3181 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
3241 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
3301 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
3361 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
3421 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
3481 gaggagcagg actgaccgac gccgaccaac accgccggtc cgacggcggc ccacgggtcc
3541 caggggggtc gacctcgaaa cttgtttatt gcagcttata atggttacaa ataaagcaat
3601 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
3661 aaactcatca atgtatctta tcatgtct
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