HPV筛查 CISH(显色原位杂交技术)探针

Abnova产品货号： CG0026
产品全称：   HPV筛查 CISH(显色原位杂交技术)探针
- 【HPV Screening CISH Probe】
产品描述：HPV筛查CISH探针用于定性检测人乳头瘤病毒(HPV)6/11/16/18/31/33/35/39/45/51/52/56/58/59/66/68/82固定,石蜡包埋标本中的DNA通过显色原位杂交技术(CISH).
- 【HPV Screening CISH Probe is designed for the qualitative detection of human papillomavirus (HPV) type 6/11/16/18/31/33/35/39/45/51/52/56/58/59/66/68/82 DNA in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).】
基因名称及描述： 
暂无说明
抗原种属： 暂无说明
适用实验样本种属： 暂无说明
承诺应用实验类型：CISH-P
注意事项： 该探针旨在与CISH实施HRP-DAB试剂盒结合使用(目录编号: KA5367 ),为样品预处理和杂交后处理提供必要的试剂.
在整合HPV的情况下,细胞核中的染色模式可以观察为离散和点状信号,或在游离HPV的情况下作为强而均匀的核染色.当检测到HPV的RNA序列时,观察到细胞质染色.信号的可视化应该使用从至少200倍到630倍放大率的一组物镜来执行.通常用200倍放大倍数的物镜清楚地检测附加型染色图案的存在,而集成HPV图案的检测需要更大的放大倍数,优选630倍.不要评估坏死区域,重叠核,过度消化的细胞核和细胞核以及弱信号强度.
- 【The probe is intended to be used in combination with the CISH Implementation HRP-DAB Kit (Catalog #: KA5367), which provides necessary reagents for specimen pretreatment and post-hybridization processing.
The staining pattern in the nucleus can be observed as discrete and dot-shaped signals in case of integrated HPV, or as a strong and homogeneous nuclear staining in case of episomal HPV. A cytoplasmic staining is observed when RNA sequences of HPV are detected. Visualization of signals should be performed using a set of objectives ranging from an at least 200-fold to 630-fold magnification. The presence of the episomal staining pattern is usually detected clearly by an objective with 200-fold magnification, whereas the detection of the integrated HPV pattern requires a greater magnification, preferably 630-fold. Do not evaluate areas of necrosis, overlapping nuclei, over-digested nuclei and nuclei with weak signal intensity.】