| Synonym: | Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase |
| CAS Number: | 9003-99-0 |
| Enzyme Commission (EC) Number: | |
| EC Number: | 232-668-6 |
| MDL number: | MFCD00071339 |
| Analysis Note | The RZ (Reinheitszahl) is the absorbance ratio A403/A275 determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity. |
| | This product is assayed using ABTS for easy comparison to other suppliers' unit: approx. 1,000 units per mg solid |
| Linkage | Similar to P8375 |
| Other Notes | View more information on peroxidase at www.sigma-aldrich.com/enzymeexplorer. |
| Packaging | Packaged in mg solid |
| Unit Definition | One ABTS unit will oxidize 1 μmole of ABTS per minute at |
| | One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at |
| General description | Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0. |
| type | Type VI-A |
| form | essentially salt-free, lyophilized powder |
| EQP level | Premium |
| storage temp. | 2 |
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
| 品名 | 产地 | 货号 | 规格 | 单价 | 备注 |
| HRP辣根过氧化物酶 | Sigma | P6782 | 10mg | 询价 | 现货 |
| HRP辣根过氧化物酶 | Sigma | P6782 | 25mg | 询价 | 现货 |
| HRP辣根过氧化物酶 | Sigma | P6782 | 50mg | 询价 | 现货 |
| HRP辣根过氧化物酶 | Sigma | P6782 | 100mg | 询价 | 现货 |
