Human Ceramide Kinase / CERK ELISA Kit (Sandwich ELISA) - LS-F9732 Catalog Size Price LS-F9732-1 1 plate $840 USD Available Discounts 10% off 5+ units Request Bulk Add to Wish List Add to CartPerformance Guaranteed SpecificationsPublicationsReviewsImagesPopular Ceramide Kinase / CERK Elisa Kits Mouse Ceramide Kinase / CERK ELISA Kit (Sandwich ELISA) - LS-F17059 Reactivity: Mouse Range: 78-5000 pg/ml Human Ceramide Kinase / CERK ELISA Kit (Sandwich ELISA) - LS-F11113 Reactivity: Human Range: 0.625-40 ng/ml Human Ceramide Kinase / CERK ELISA Kit (Sandwich ELISA) - LS-F35170 Reactivity: Human Range: 0.625-40 ng/mlProduct DescriptionLS-F9732 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human Ceramide Kinase / CERK in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Ceramide Kinase / CERK as low as 1.17 nanograms per millilter. More Ceramide Kinase / CERK Products Top of PageSpecificationsType
Ceramide Kinase (CERK) No significant cross-reactivity or interference between Ceramide Kinase (CERK) and analogs was observed. Manual
Intended Sample Types
Cell Lysates, Tissue Homogenates Format
96-Well Strip Plate Detection
Colorimetric - 450nm (TMB) Measurement
Quantitative Detection Range
3.13 - 200 ng/ml Sensitivity
1.17 ng/ml Assay Time
4.5 h Precision
Intra-Assay: CV<10% Inter-Assay: CV<12% Storage
Short term: 4°C; Long term: see manual. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
Coated 96-well Strip Plate
Adhesive Plate Sealers
Standard (Lyophilized)
Sample Diluent
Detection Reagent A (Green)
Detection Reagent B (Red)
Assay Diluent A
Assay Diluent B
TMB Substrate
Stop Solution
Wash Buffer (30x concentrate)
Restrictions
For research use only. Guarantee
This elisa kit carries the LSBio Guarantee. About Ceramide Kinase / CERK
CERK, which phosphorylates Cer to produce ceramide 1-phosphate (C1P), was initially described as a Ca2+-stimulated lipid kinase that copurified with brain synaptic vesicles. CERK activity has been reported in HL60 cells, mast cells, and neutrophils. CERK is involved in phagolysosome formation in polymorphonuclear leukocytes and also in liposome fusion. C1P has been reported to have mitogenic effects. More recently, C1P was found to induce arachidonic acid release and prostanoid synthesis. Q8TCT0 NM_022766 NP_073603.2
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