测序及更多信息：https://pan.baidu.com/s/1vpXgtug_TKN1a_lhVz8WUQ 密码：o6dv
采购地址：淘宝店铺地址
pmir-Glo载体基本信息
| 出品公司: | Promega |
| 载体名称: | pMir-Glo, pmirGLO |
| 质粒类型: | miRNA功能研究载体，荧光素酶报告系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | PGK |
| 载体大小: | 7350bp |
| 3' 测序引物及序列: | PCMV-R TCCAAACTCATCAATGTATC |
| 载体标签: | N-Luc2 |
| 载体抗性: | 氨苄 |
| 稳定性: | 瞬时表达 |
| 组成型: | 非组成型 |
全序列
1 AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT
61 TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG
121 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG
181 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG
241 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC
301 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG
361 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
421 AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA
481 CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT
541 TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC
601 ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA
661 GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG
721 CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG
781 CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT
841 CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC
901 GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT
961 GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC
1021 CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC
1081 TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG
1141 ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA
1201 GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG
1261 ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG
1321 GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC
1381 AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT
1441 CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA
1501 GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG
1561 GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC
1621 GAGCAGTAAT TCTAGGCGAT CGCTCGAGCC CGGGAATTCG TTTAAACCTA GAGCGGCCGC
1681 TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAGGA
1741 TCTAAATGAG TCTTCGGACC TCGCGGGGGC CGCTTAAGCG GTGGTTAGGG TTTGTCTGAC
1801 GCGGGGGGAG GGGGAAGGAA CGAAACACTC TCATTCGGAG GCGGCTCGGG GTTTGGTCTT
1861 GGTGGCCACG GGCACGCAGA AGAGCGCCGC GATCCTCTTA AGCACCCCCC CGCCCTCCGT
1921 GGAGGCGGGG GTTTGGTCGG CGGGTGGTAA CTGGCGGGCC GCTGACTCGG GCGGGTCGCG
1981 CGCCCCAGAG TGTGACCTTT TCGGTCTGCT CGCAGACCCC CGGGCGGCGC CGCCGCGGCG
2041 GCGACGGGCT CGCTGGGTCC TAGGCTCCAT GGGGACCGTA TACGTGGACA GGCTCTGGAG
2101 CATCCGCACG ACTGCGGTGA TATTACCGGA GACCTTCTGC GGGACGAGCC GGGTCACGCG
2161 GCTGACGCGG AGCGTCCGTT GGGCGACAAA CACCAGGACG GGGCACAGGT ACACTATCTT
2221 GTCACCCGGA GGCGCGAGGG ACTGCAGGAG CTTCAGGGAG TGGCGCAGCT GCTTCATCCC
2281 CGTGGCCCGT TGCTCGCGTT TGCTGGCGGT GTCCCCGGAA GAAATATATT TGCATGTCTT
2341 TAGTTCTATG ATGACACAAA CCCCGCCCAG CGTCTTGTCA TTGGCGAATT CGAACACGCA
2401 GATGCAGTCG GGGCGGCGCG GTCCCAGGTC CACTTCGCAT ATTAAGGTGA CGCGTGTGGC
2461 CTCGAACACC GAGCGACCCT GCAGCGACCC GCTTAAAAGC TTGGCATTCC GGTACTGTTG
2521 GTAAAGCCAC CATGGCCGAT GCTAAGAACA TTAAGAAGGG CCCTGCTCCC TTCTACCCTC
2581 TGGAGGATGG CACCGCTGGC GAGCAGCTGC ACAAGGCCAT GAAGAGGTAT GCCCTGGTGC
2641 CTGGCACCAT TGCCTTCACC GATGCCCACA TTGAGGTGGA CATCACCTAT GCCGAGTACT
2701 TCGAGATGTC TGTGCGCCTG GCCGAGGCCA TGAAGAGGTA CGGCCTGAAC ACCAACCACC
2761 GCATCGTGGT GTGCTCTGAG AACTCTCTGC AGTTCTTCAT GCCAGTGCTG GGCGCCCTGT
2821 TCATCGGAGT GGCCGTGGCC CCTGCTAACG ACATTTACAA CGAGCGCGAG CTGCTGAACA
2881 GCATGGGCAT TTCTCAGCCT ACCGTGGTGT TCGTGTCTAA GAAGGGCCTG CAGAAGATCC
2941 TGAACGTGCA GAAGAAGCTG CCTATCATCC AGAAGATCAT CATCATGGAC TCTAAGACCG
3001 ACTACCAGGG CTTCCAGAGC ATGTACACAT TCGTGACATC TCATCTGCCT CCTGGCTTCA
3061 ACGAGTACGA CTTCGTGCCA GAGTCTTTCG ACAGGGACAA AACCATTGCC CTGATCATGA
3121 ACAGCTCTGG GTCTACCGGC CTGCCTAAGG GCGTGGCCCT GCCTCATCGC ACCGCCTGTG
3181 TGCGCTTCTC TCACGCCCGC GACCCTATTT TCGGCAACCA GATCATCCCC GACACCGCTA
3241 TTCTGAGCGT GGTGCCATTC CACCACGGCT TCGGCATGTT CACCACCCTG GGCTACCTGA
3301 TTTGCGGCTT TCGGGTGGTG CTGATGTACC GCTTCGAGGA GGAGCTGTTC CTGCGCAGCC
3361 TGCAAGACTA CAAAATTCAG TCTGCCCTGC TGGTGCCAAC CCTGTTCAGC TTCTTCGCTA
3421 AGAGCACCCT GATCGACAAG TACGACCTGT CTAACCTGCA CGAGATTGCC TCTGGCGGCG
3481 CCCCACTGTC TAAGGAGGTG GGCGAAGCCG TGGCCAAGCG CTTTCATCTG CCAGGCATCC
3541 GCCAGGGCTA CGGCCTGACC GAGACAACCA GCGCCATTCT GATTACCCCA GAGGGCGACG
3601 ACAAGCCTGG CGCCGTGGGC AAGGTGGTGC CATTCTTCGA GGCCAAGGTG GTGGACCTGG
3661 ACACCGGCAA GACCCTGGGA GTGAACCAGC GCGGCGAGCT GTGTGTGCGC GGCCCTATGA
3721 TTATGTCCGG CTACGTGAAT AACCCTGAGG CCACAAACGC CCTGATCGAC AAGGACGGCT
3781 GGCTGCACTC TGGCGACATT GCCTACTGGG ACGAGGACGA GCACTTCTTC ATCGTGGACC
3841 GCCTGAAGTC TCTGATCAAG TACAAGGGCT ACCAGGTGGC CCCAGCCGAG CTGGAGTCTA
3901 TCCTGCTGCA GCACCCTAAC ATTTTCGACG CCGGAGTGGC CGGCCTGCCC GACGACGATG
3961 CCGGCGAGCT GCCTGCCGCC GTCGTCGTGC TGGAACACGG CAAGACCATG ACCGAGAAGG
4021 AGATCGTGGA CTATGTGGCC AGCCAGGTGA CAACCGCCAA GAAGCTGCGC GGCGGAGTGG
4081 TGTTCGTGGA CGAGGTGCCC AAGGGCCTGA CCGGCAAGCT GGACGCCCGC AAGATCCGCG
4141 AGATCCTGAT CAAGGCTAAG AAAGGCGGCA AGATCGCCGT GTAATAATTC TAGAGTCGGG
4201 GCGGCCGGCC GCTTCGAGCA GACATGATAA GATACATTGA TGAGTTTGGA CAAACCACAA
4261 CTAGAATGCA GTGAAAAAAA TGCTTTATTT GTGAAATTTG TGATGCTATT GCTTTATTTG
4321 TAACCATTAT AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC
4381 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA
4441 AAATCGATAA GGATCCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT
4501 TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC
4561 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC
4621 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA
4681 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG
4741 GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG
4801 TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC
4861 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA
4921 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG
4981 CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA
5041 ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC
5101 CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT
5161 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG
5221 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA
5281 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA
5341 AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA
5401 ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG
5461 TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG
5521 TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT
5581 GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG
5641 TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC
5701 AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA
5761 CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA
5821 CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC
5881 TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG
5941 GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC
6001 AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG
6061 TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT
6121 ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT
6181 CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
6241 CCTTTTGCTG GCCTTTTGCT CACATGGCTC GAC
