PowerUp™ SYBR™ Green Master Mix

PowerUp SYBR Green 预混液适用于所有qPCR仪器的通用型预混液

PowerUp™ SYBR™ Green 预混液的特性包括：

• 双重热启动机制提供了出众的特异性
• 在较宽的动态范围内都能获得可重复性的 C_t 值
• 适用于标准或快速循环，<50分钟内即可获得结果
• 采用 UN G和 dUT P配方，可防止残留 PCR 产物污染
• 制备好qPCR反应板后，可以在室温下稳定保存72小时
• 兼容多种仪器

货号：  A25778
 相关应用： 实时定量 PCR


PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR® Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

PowerUp™ SYBR® Green Master Mix features include:
• A dual hot-start mechanism for excellent specificity
• Highly reproducible CTs over a broad dynamic range
• Inclusion of UDG to help prevent carryover contamination
• Stability of pre-assembled reactions for up to 72 hours
• Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism
Specificity is paramount in obtaining high-quality data in SYBR® Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR® Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR® Green Master Mix generated single melt curves of the time, consistent with highly specific amplification.

Tight reproducibility in CTs over a wide dynamic range
PowerUp™ SYBR® Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested (see figure). In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR® Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality (see figure).

UDG included for carryover contamination control
The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR® Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.

Stability of pre-assembled reactions
The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR® Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data (see figure). This consistency allows users to have confidence and flexibility in the use of PowerUP™ SYBR® Green Master Mix across numerous workflow scenarios¹.

Broad instrument compatibility
PowerUp™ SYBR® Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments2. It is also compatible with the Bio-Rad IQ™5 and CFX96™ / CFX384™ systems, Roche LightCycler® LC480, and Stratagene® MX3005P® systems. For optimal results, recommended primer concentrations are 300–800 nM.



 

Detection Method:	SYBR
For Use With (Equipment):	7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Reaction Speed:	Fast or Standard
PCR Method:	qPCR
Sample Type (General):	DNA
Number of Reactions:	5000 reactions
Polymerase:	Dual-Lock™ Taq DNA Polymerase
Product Line:	PowerUP™, SYBR®
Product Size:	10 x 5 mL
Shipping Condition:	Wet Ice

内容及储存The 2x mix contains SYBR Green dye, Dual-Lock Taq DNA Polymerase, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye, and optimized buffer components. Contains 10 X 5 mL tube, sufficient for 5000 20-µL reactions (10 µL Master Mix per reaction).

Store at 2–8°C.
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