3T6-Swiss albino细胞系——小鼠胚胎成纤维细胞系如何传代

3T6-Swiss albino细胞系——小鼠胚胎成纤维细胞系如何传代 货号：GD-C11419226

细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
公司主营ATCC、DSMZ、RIKEN、ScienCell、INCELL、Promocell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国内外大学来源细胞系及原代细胞，全程提供细胞系背景、生长特性、来源、器官、类型、形态、培养条件、应用、系、疾病、组织、冻存条件、传代方法等复苏及冻存说明书信息，我们拥有丰富的细胞系培养经验，能提供细胞培养全方位的技术支持，让您实验再无烦扰！公司由在国外工作和学习多年的留学人员创办，由国内ZG科学院上海生物化学与细胞生物学研究所，复旦大学，同济大学等多名工作在线的细胞培养专家，提供细胞培养技术支持。公司依靠自身一些过硬的技术和理念，以世界的技术支撑阵容，提供一系列细胞相关的技术服务。为ZG的生命科学事业赶超欧美，提供了重要的基石；公司将依靠自主研究开发的高技术高质量和有的价格竞争力的产品，立足国内，面向世界。力争打破国外大公司在细胞培养科学领域接近垄断的地位，为ZG在生命科学的这一领域占领一席之地。公司筹划未来三年内，在沈阳、济南、北京、西安、宁夏、武汉、重庆等国内代表城市建立细胞培养分实验室，以便更好的服务国内广大医院、大学、制药企业等，你们的每一份关心和支持，都是我们前进的动力！
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
购买的细胞死亡或细胞存活率不佳可能原因？研究人员在细胞培养时出现存活率不佳，原因比较复杂，常见原因可归纳为：培养基使用错误或培养基品质不佳；血清使用错误或血清的品质不佳；解冻过程错误；冷冻细胞解冻后，加以洗涤细胞和离心；悬浑细胞误认为死细胞；培养温度使用错误；细胞置于-80℃太久等。建议严格参照ATCC的标准操作规程进行细胞复苏、冻存等工作。欲将一般动物细胞离心下来，其离心速率应为多少转速?欲回收动物细胞，其离心速率一般为300×g(约1OOOrpm),5-10分钟，转速过高或离心时间过长都将造成细胞死亡。合适的离心转速是根据相对离心力决定。RCF=1.119×105×r×(rpm)2，其中r为离心机转轴ZX与离心套管底部内壁的距离；rpm为离心机每分钟的转数；RCF(relative centrifugal force)为相对离心力，以重力加速度g(980.66cm/s2)的倍数来表示单位。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
Methyl 3,4,5-trihydroxybenzoate, 98%
TyraMine
4'-CYANOBENZYLIDENE-4-ETHOXYANILINE
5-(TrifluoroMethyl)dibenzothiopheniuM tetrafluoroborate
1,1′-Bis{(R)-{(RP)-2-[(S)-1-(diMethylaMino)ethyl]ferrocenyl}phenylphosphino}ferrocene
6-Methylpyridine-2-carboxylic acid hydrochloride hydrate
trans-Chalcone
1-Benzothiophen-5-ylmethanol, 97%
3-[(3,5-dimethyl-1H-pyrazol-1-yl)methyl]benzoic acid(SALTDATA: FREE)
5-Chloro-2-fluorophenylacetic acid
Zinc sulfate monohydrate, 99%
N-(tert-Butoxycarbonyl)-O-benzyl-D-threonine
2,4-Bis(trifluoromethyl)benzyl bromide
Tetracaine
alpha-CyclopentylMandelic acid
4-(TrifluoroMethyl)benzoyl chloride
3,4-Dehydro-L-proline
6-Benzyloxyindole-3-carboxaldehyde
Xylidine Ponceau 2R, pure
Sorbitan Monostearate, NF
Azocal A [for CalciuM deterMination]
stearonitrile
trans-4-Methylcyclohexylamine
4-Nitrobenzophenone
N-Cinnamoyl-β-hydroxy-4-methoxyphenyl-aethylamin
6-AMINOCHRYSENE
3,4-Dichloroiodobenzene
1,8-Octanediol
4-Fluoro-N-isopropylbenzylaMine, 97%
4-(1-Piperidinylsulfonyl)benzeneboronic acid, 97%
SeleniuM
3-BroMo-2-Methoxybenzoic acid
4-Piperidineethanol
Oxindole-5-boronic acid, pinacol ester
1,3-DICHLORO-4-IODOBENZENE
1-BroMotetradecane
Oxalyl Chloride
5-Bromo-m-xylene
2,6-Dimethoxypyrimidine-4-carboxylic acid
Biphenyl-4-glyoxal hydrate 95%
2-Pyrazinylethanethiol
3-(2-Phenyl-1H-imidazol-1-yl)propanenitrile
1,1-DIFLUOROETHYLENE
Nalpha-Cbz-L-Arginine
Methyl 1,2,3-benzothiadiazole-5-carboxylate
4-Acetylpyridine
D-Histidine Methyl Ester Dihydrochloride
N-Boc-3-piperidineacetic acid
N-BOC-4-oxo-L-proline, 98%
N-Cbz-D-glutamic acid alpha-benzyl ester
6-BroMo-o-cresol
4-Iodo-m-xylene
1-hydroxy-1-cyclopropanecarboxylic acid
pentyl phenylacetate
p-Bromophenoxyacetic acid
5-Fluoro-2-methylphenylhydrazine hydrochloride
2-(TRIFLUOROMETHYL)THIOPHENOL
PotassiuM hexafluorotitanate
1,2-PHENYLENE PHOSPHOROCHLORIDITE
3-Bromophenethylamine, 99%
3,5-Dimethoxybenzyl Alcohol
Tetrakis[(R)-(-)-(1-adamantyl)-(N-phthalimido)acetato]dirhodium(II) Rh2(R-PTAD)4
4-(Trifluoromethyl)quinolin-2-amine ,97%
6-METHOXY-1H-INDOLE-2-CARBOXYLIC ACID
2-METHOXYPHENYL ISOTHIOCYANATE
1-Iodo-2-(trifluoromethoxy)benzene
Pentachloroethane;
3-(1-Azepanyl)-2,2-diMethylpropylaMine
1-BroMo-3-(difluoroMethoxy)benzene
2-Hydroxy-3-Methylphenylboronic acid
DL-2-Methylbutyric acid, 98%
3-(CyanoMethyl)phenylboronic acid pinacol ester, 97%
1,3-DiMethoxy-1,1,3,3-tetraphenyldisiloxane
4-EthylcyclohexylaMine (cis- and trans- Mixture)
(2-chloroethyl)sulphonyl chloride
N-Lauroyl-D-arginine
(2R,4R)-3-Benzyloxycarbonyl-4-methyl-2-phenyl-1,3-oxazolidin-5-one
acetyl dihydrogen phosphate, lithium potassium salt
MBP ELISA Kit
Salusin B ELISA Kit
Human FBN2 (Fibrillin 2) ELISA Kit
Human UBC ELISA Kit
Chicken β-lactamase ELISA Kit
α-SYN ELISA Kit
Chicken Thyroxine,T4 ELISA Kit
ChAT ELISA Kit
Human alpha-granular membrane protein,GMP-140 ELISA Kit
PAMY ELISA Kit
Gentamicin Agar Base
Eugon LT 100 Broth Base
Methyl acetoacetate
AntiMony(III) fluoride, 98%
5,6-Diphenyl-3-(2-pyridyl)-1,2,4-triazine-4,4"-disulfonic acid MonosodiuM salt hydrate, 97%
2-Fluoro-4-Methoxybenzyl Chloride
stearonitrile
Tungsten(VI) chloride
isophorone oxide
1-Chloro-2-fluoro-4-methoxybenzene

4-Hydroxybenzyl cyanide
CalciuM Hydride
6-Oxa-1-azaspiro[3.3]heptane, 95%
4-(BenzylcarbaMoyl)benzeneboronic acid, 98%
DiethylMethoxyborane, 1M soln in THF
SodiuM phenoxyacetate
PotassiuM sulfite
SALICYLALDEHYDE HYDRAZONE
DiMethyl fuMarate
6-CHLOROTHIOCHROMAN-4-ONE
BARIUM NIOBIUM OXIDE
Ethyl 1-benzylpiperidine-4-carboxylate 96%
DL-(4-Chlorophenyl)glycine
5-Amino-3-methyl-4-isoxazolecarbonitrile
Lithium phenoxide, 0.6M solution in THF, AcroSeal