HeLa229细胞：人宫颈癌复苏快细胞系

HeLa229细胞：人宫颈癌复苏快细胞系 货号：GD-C4720071
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
接收细胞相关问题：如不能在收到细胞后及时操作细胞，T25及离心管发货的细胞将可以消毒后在37度过夜，血清发货的细胞在室温下静置1-2天(注意不要放冰箱)，干冰发货的可以在确认干冰未完全升华时将细胞直接放回液氮或者-80度冰箱，若干冰完全升华，请即刻复苏细胞。T25瓶及离心管在培养箱平衡是为了让细胞尽快适应培养箱环境，同时使部分因运输导致脱落的细胞贴壁，此步骤非常必要。T25瓶如果在显微镜下可以看到部分不贴壁的细胞，可以收集上清后离心(1200rpm，5min)后，将沉淀用新的培养基重悬后接种至新的培养瓶或皿中。部分细胞在运输过程中，由于不断震荡及环境不适，可能导致细胞破碎死亡，从而使培养基中漂浮很多细胞碎片及颗粒状物质。这种情况下，平衡后，贴壁细胞用PBS洗两遍再加新的培养基培养或者传代至新瓶中培养即可；悬浮细胞可以离心(1000rpm，3min)收集细胞，并用PBS重悬后再离心一次，再用新的培养基重悬并接种细胞。
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
2-Fluorotoluene, 99+%
2,5-DibroMo-3-butylthiophene
(S)-(+)-4-Phenyl-2-oxazolidinone
(3R)-(-)-1-BENZYL-3-(ETHYLAMINO)PYRROLIDINE
3-(BROMOMETHYL)QUINOXALIN-2(1H)-ONE
ETHYL HEPTAFLUOROBUTYRATE
2,2-DiMethylvaleronitrile
2,4-Dichlorobenzyl Mercaptan
N-Boc-2-(3-trifluoromethyl-phenyl)-DL-glycine
4-(chloromethyl)benzamide(SALTDATA: FREE)
2,2-Dichloropentanoic acid, 92%
D-GLUCURONIC ACID SODIUM SALT
Mestanolone
4-(Trifluoromethyl)benzene-1-sulfonyl chloride
2-Methylcyclohexylamine
4-Fluoro-2-(trifluoroMethyl)benzenesulfonyl chloride
2,5-DiMethoxy-3-nitrobenzoic Acid
2,5-DibroMo-3-chloropyridine
Thorin I, pure, indicator grade
Paclitaxel, USP
N,N',N''-Triphenyl-1,3,5-benzenetriaMine
1-(2-Aminophenyl)-ethanone
N-isopropylhydroxylamine
N-Isopropylaniline
4',4'''-azobis[[1,1'-biphenyl]-4-carboxylic] acid
Birch-Me
Barbituric acid
Zinc Silicate
2-Chloro-3-(difluoroMethyl)quinoline, 97%
Methyl 3-chloro-4-hydroxybenzoate, 98%
7-Nitro-1,2,3,4-tetrahydroisoquinoline hydrochloride, 97+%
9,10-Bis(phenylethynyl)anthracene
4-METHYLBENZYL MERCAPTAN
Salicylic acid
Dichloroacetonitrile
4-HYDROXY-CYCLOHEXANECARBOXYLIC ACID ETHYL ESTER
p-Terphenyl
1-Ethynylcyclohexanol
2,3-Dimethylhexane
Benzyl 4-(aminomethyl)tetrahydro-1(2H)-pyridinecarboxylate, 97%
2-(2-Diethylaminoethoxy)ethyl 1-phenylcyclopentanecarboxylate citrate
Tetrasodium pyrophosphate
trans-1,4-Diaminocyclohexane
(R)-(-)-2-HEXANOL
CesiuM fluoride
3-(ChloroMethyl)-5-(3,5-diMethylisoxazol-4-yl)-1,2,4-oxadiazole
4,4'-DiMethoxy-2,2'-bipyridyl
Methyl 3-broMo-5-hydroxybenzoate
Zinc oxide, ACS reagent
StrontiuM Carbonate, Powder, Reagent
2-Fluoro-6-nitrotoluene
erythromycin stearate
2-Hydroxy-3-nitropyridine
Z-L-methionine
1-(4-fluorophenyl)-2-thiourea
3,5-Dimethoxybenzoic acid
5-Fluoro-2-Methoxycarbonylphenylboronic acid
2-BROMO-4-CYANO-6-METHOXYPHENYL ACETATE
1,4-BIS(2-METHYLSTYRYL)BENZENE
4-Aminoquinazoline
4,4,5,5,6,6,6-Heptafluorohexanoic acid
2,4-Dihydroxybenzaldehyde ,98%
2-Bromo-1-(1,3-dimethyl-1H-pyrazol-5-yl)ethanone ,90%
Benzilic acid
7-Diethylamino-4-methylcoumarin
1,5-HEXADIYNE
Octabenzone
2-AMinoterephthalic acid
1,3-Bis(4-aMinophenoxy)benzene
4-(CarboxyMethoxy)phenylboronic acid
AnthranilaMide, 99+%
1-tert-Butyl 4-(9-H-fluoren-9-ylMethyl) hydrogen (2S)--1,2,4-tricarboxyl, 97%
cis-6-Nonen-1-ol
3-Ethoxysalicylaldehyde
2,5-Diiodothiophene
(S)-1-Benzyl-3-Aminopiperidine
3-bromotetrahydro-4H-pyran-4-one
Esfenvalerate
Tβ4 ELISA Kit
BACE 2 ELISA Kit
Human lipoprotein lipase，LPL ELISA Kit
Human replication protein A,RPA-70 ELISA Kit
HSP-20 ELISA Kit
JAK2 ELISA Kit
LEP ELISA Kit
Human vanillylmandelic acid,VMA ELISA Kit
Human Bromodeoxyuridine,BrdU ELISA Kit
VD ELISA Kit
Bismuth Sulfite Glucose Glycine Yeast Agar
Biotin Assay Medium
Indole-2-carboxaldehyde, 97%
BariuM chroMate, 98%
5-AMino-3,4-diMethylisoxazole, 99%
2-Methyl-1-naphthylMagnesiuM broMide, 0.25 M in 2-MeTHF
1-(2-Aminophenyl)-ethanone
Cadmium sulfate
Methyl 4-chlorocinnamate
2-Amino-6-purinethiol

(S)-2-(Aminomethyl)-1-ethylpyrrolidine
Fluorescein
DiphenylaMine-4-Sulfonic Acid BariuM Salt
N-Boc-3-fluoro-L-phenylalanine, 95%
Pentafluorobenzene, 98+%
4-BroMobiphenyl, 98+%
(±)-sec-ButylaMine
Lead(II) Metaborate Monohydrate
2-(5-BROMO-2-PYRIDYLAZO)-5-DIMETHYLAMINOPHENOL
4,4'-DIFLUOROBENZYLIDENEANILINE
Neroli oil, Morocco
4-(CyclopropylaMinocarbonyl)phenylboronic acid
1-ethyl-3,5-dimethyl-1H-pyrazole(SALTDATA: FREE)
4-Hydrazinobenzonitrile hydrochloride
Ethyl 4-Nitropyrrole-2-carboxylate