Anti-phospho-MARCKS (Ser152 Ser156)

存储温度：Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

来源：Rabbit, IgG

存储溶液：100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.

参考文献：1. Ouimet, C C. et al: L°C alization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. 1990 J Neurosci 10:1683-1698. 2. Hartwig J H. et al: MARCKS is an a ctin filament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 1993356: 618-622. 3. Stumpo D J. et al: MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Pr°C . Nat. Acad. Sci. 1995 92; 944-948. 4. Verghese G M. et al: Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem 1994 269:9361-9367.

特异性：Recognizes the MARKS protein phosphorylated at serine 152 and serine 156

应用：WB

官网链接：https://www.signalchem.com/product_details.php?id=4744

商品关键词：Anti-phospho-MARCKS (Ser152 Ser156),M02-565R-100,SignalChem

简单描述：Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a member of a family of calmodulin binding proteins thatis a target for phosphorylation by protein kinase C (PKC) (1). MARCKS is concentrated in the synapses of neurons where it appears to function in synaptic vesicle cycling. MARCKS has been shown to bind both actin and calmodulin in vitro (2). Deletion of the MARCKS gene in mice results in embryonic brain defects and death (3). Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (4).

Cross Reactivity: Human, Mouse, Rat, Bovine, Chicken, Xenopus and Zebrafish

Purification: Affinity Chromatography

Sample Data: Western blot of rat brain lysate showing specific immunolabeling of the ~87kDa MARCKS protein phosphorylated at Ser152/156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-MARCKS (Ser152 Ser156) antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.


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