Rat Adiponectin ELISA Kit (Sandwich ELISA) - LS-F10043 Catalog Size Price LS-F10043-1 1 plate $800 USD Available Discounts 10% off 5+ units Request Bulk Add to Wish List Add to CartPerformance Guaranteed SpecificationsPublicationsReviewsImagesPopular Adiponectin Elisa Kits Rabbit Adiponectin ELISA Kit (Sandwich ELISA) - LS-F5126 Type: Sandwich ELISA Format: 96-Well Strip Plate Reactivity: Rabbit Range: 0.313-20 ng/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Serum, Plasma Pig Adiponectin ELISA Kit (Sandwich ELISA) - LS-F5346 Type: Sandwich ELISA Format: 96-Well Strip Plate Reactivity: Pig Range: 1.56-100 ng/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Serum, Plasma Bovine Adiponectin ELISA Kit (Sandwich ELISA) - LS-F6080 Type: Sandwich ELISA Format: 96-Well Strip Plate Reactivity: Bovine Range: 0.781-50 ng/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Serum, Plasma Human Adiponectin ELISA Kit (Sandwich ELISA) - LS-F22450 Type: Quantitative Sandwich ELISA Format: 96-Well Microplate Reactivity: Human Range: 0.78-50 ng/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Serum, Plasma Human Adiponectin ELISA Kit (Sandwich ELISA) - LS-F24347 Type: Quantitative Sandwich ELISA Format: 96-Well Strip Plate Reactivity: Human Range: 62.5-4000 pg/ml Detection: Colorimetric - 450nm (TMB) Sample Types: Cell Culture Supernatants, Serum, Tissue Homogenates, PlasmaProduct DescriptionLS-F10043 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat Adiponectin in samples of Cell Culture Supernatants, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Adiponectin as low as 0.039 nanograms per millilter. About Adiponectin Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Q15848 NM_004797 NP_004788.1
This assay has high sensitivity and excellent specificity for detection of rat ADP. No significant cross-reactivity or interference between rat ADP and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between rat ADP and all the analogs, therefore, cross reaction may still exist. Manual
Short term: 4°C; Long term: see manual. Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Kit Components
Assay plate (12 x 8 coated Microwells)
Standard (Lyophilized)
Biotin-antibody (100 x concentrate)
HRP-avidin (100 x concentrate)
Biotin-antibody Diluent
HRP-avidin Diluent
Sample Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip (For 96 wells)
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADP bound in the initial step. The color development is stopped and the intensity of the color is measured. Restrictions
For research use only. Guarantee
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