Mouse Adiponectin ELISA Kit (Sandwich ELISA) - LS-F10044

Mouse Adiponectin ELISA Kit (Sandwich ELISA) - LS-F10044
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LS-F10044-1
1 plate
$800 USD
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SpecificationsPublicationsReviewsImagesPopular Adiponectin Elisa Kits
Rabbit Adiponectin ELISA Kit (Sandwich ELISA) - LS-F5126
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rabbit
Range: 0.313-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Pig Adiponectin ELISA Kit (Sandwich ELISA) - LS-F5346
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Pig
Range: 1.56-100 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Bovine Adiponectin ELISA Kit (Sandwich ELISA) - LS-F6080
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Bovine
Range: 0.781-50 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Human Adiponectin ELISA Kit (Sandwich ELISA) - LS-F22450
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Human
Range: 0.78-50 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Human Adiponectin ELISA Kit (Sandwich ELISA) - LS-F24347
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 62.5-4000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Tissue Homogenates, Plasma

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Product DescriptionLS-F10044 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse Adiponectin in samples of Cell Culture Supernatants, Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Adiponectin as low as 0.039 nanograms per millilter.
About Adiponectin
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Uniprot: Q15848 NCBI: NM_004797 NP_004788.1

Synonyms: ADIPOQ ElisaKit, Adiponectin ElisaKit, ADPN ElisaKit, ACDC ElisaKit, ADIPQTL1 ElisaKit, APM-1 ElisaKit, APM1 ElisaKit, ACRP30 ElisaKit, Gelatin-binding protein 28 ElisaKit, GBP28 ElisaKit, Gelatin-binding protein ElisaKit

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SpecificationsType
 
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
 
Adiponectin
Reactivity
 
Mouse
Specificity
 
This assay has high sensitivity and excellent specificity for detection of mouse ADP. No significant cross-reactivity or interference between mouse ADP and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between mouse ADP and all the analogs, therefore, cross reaction may still exist.
Manual
 

Intended Sample Types
 
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
Sample Size
 
50 - 100 µl
Format
 
96-Well Microplate
Detection
 
Colorimetric - 450nm (TMB)
Measurement
 
Quantitative
Detection Range
 
0.156 - 10 ng/ml
Sensitivity
 
0.039 ng/ml
Precision
 
Intra-assay: CV%<8% Inter-assay: CV%<10%
Storage
 
Short term: 4°C; Long term: see manual.
Quality Assurance
 
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
 

• Assay plate (12 x 8 coated Microwells)
• Standard (Lyophilized)
• Biotin-antibody (100 x concentrate)
• HRP-avidin (100 x concentrate)
• Biotin-antibody Diluent
• HRP-avidin Diluent
• Sample Diluent
• Wash Buffer (25 x concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip (For 96 wells)

Background
 
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Restrictions
 
For research use only.
Guarantee
 
This elisa kit carries the LSBio Guarantee.
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