动物体内转染试剂

方法简单

如图1所示，只需要将核酸和转染试剂分别稀释，混合，无需过滤，即可直接注射动物。

转染效果

        将Entranster^TM-in vivo与Ambion in vivo siRNA(作用于凝血因子VII)和阴性siRNA通过尾静脉注射成年小鼠，2天后，取动物肝脏检测。在mRNA水平和蛋白水平观察干扰效果。见上图，图中最左侧组为对照组注射阴性siRNA为3mg/kg，后边3组为每kg动物注射阳性siRNA量分别为1mg/kg,2mg/kg和3mg/kg情况。

各器官转染

     根据推荐用量注射Entranster^TM-in vivo和siRNA（作用于Lamin A/C）和阴性siRNA到成年小鼠。注射后2天收集相应的组织，分离RNA，用qRT-PCR分析Lamin A/C基因的表达水平。图4为尾静脉结果，图5为各器官分别局部注射结果。

1.Plos one, 2016 March 9. MiR-3162-3p Is a Novel MicroRNA That Exacerbates Asthma by Regulating β-Catenin.( MiR-3162-3p通过调控β-链蛋白从而加剧哮喘的发作)

2.Arthritis Rheumatol, 2016 Apr. In Vivo Therapeutic Success of MicroRNA‐155 (miR‐155) Antagomir in a Mouse Model of Lupus Alveolar Hemorrhage.(miR‐155拮抗剂体内成功ZL狼疮性肺泡出血模型的小鼠）

3.Int J Clin Exp Patho, 2016, March 15. Regulation of ZNF580 in pathogenesis of ischemic cerebrovascular disease accompanied with upregulation of Smad2 and ADNP( 在伴随着Smad2和ADNP上调缺血性脑血管病的发病机制中ZNF580的调节）

4.Med Sci Monit. 2016 Apr 7. MiR-206 Attenuates Denervation-Induced Skeletal Muscle Atrophy in Rats Through Regulation of Satellite Cell Differentiation via TGF-β1, Smad3, and HDAC4 Signaling.(miR-206通过TGF-β1、Smad3和HDAC4信号调控卫星细胞分化进而减弱大鼠骨骼肌萎缩）

5.Oncotarget, 2015 April 20. Macrophages promote benzopyrene-induced tumor transformation of human bronchial epithelial cells by activation of NF-κB and STAT3 signaling in a bionic airway chip culture and in animal models.（巨噬细胞通过在仿生气道芯片培养和动物模型的κB和STAT3信号通路的活化从而促进苯并芘诱导的人支气管上皮细胞肿瘤的转化）(IF:6.359 )

6.Hepatology, 2015 JUL 3. MicroRNA-125b attenuates epithelial-mesenchymal transitions and targets stem-like liver cancer cells through small mothers against decapentaplegic 2 and 4.(（MicroRNA-125b通过small mothers减少Dpp2和4以减弱上皮细胞-间充质细胞转化并作用于肝癌细胞干细胞）(IF: 11.711.)

7.Critical Care Medicine, 2015, June 27. Cyclophilin A/Cluster of Differentiation 147 Interactions Participate in Early Brain Injury After Subarachnoid Hemorrhage in Rats.(大鼠蛛网膜下腔出血早期亲环蛋白A/CD147相互作用)(IF: 7.422.)

8.Cellular and Molecular Life Sciences, 2015 June 26. Rab5-mediated VE-cadherin internalization regulates the barrier function of the lung microvascular endothelium.(高危大鼠角膜移植片排斥中CD25siRNA基因转移作用)(IF: 5.808.)

9.Graefes Arch Clin Exp Ophthalmol, 2015 May 31. Effects of CD25siRNAgene transfer on high-risk rat corneal graft rejection.(高危大鼠角膜移植片排斥中CD25siRNA基因转移作用)

10.Brain behavior and immunity, 2015 April 29. MicroRNA let-7c-5p protects against cerebral ischemia injury via mechanisms involving the inhibition of microglia activation.(MicroRNA let-7c-5p通过YZ小胶质细胞活化防止脑缺血损伤) (IF: 5.889.)

11.Basic Research in Cardiology, 2015 April 10, 110:28. Sialyltransferase7A, a Klf4-responsive gene, promotes cardiomyocyte apoptosis during myocardial infarction.(Sialyltransferase7A，一个Klf4的应答基因，在心肌梗死中促进心肌细胞凋亡)(IF: 5.414.)