27291 QIAprep 96 Plus Miniprep Kit;Qiagen 27291;

产地：Qiagen货号：27291ApplicationsPlasmid DNA purified using QIAprep 96 Plus Miniprep and BioRobot Kits is highly suitable for a wide range of applications, including: Preparation of short hairpin vectors (sh-vectors) Enzymatic modifications Cloning Restriction digestion Sequencing In vitro transcription/translation Transfection into robust cell lines Principle and procedureQIAprep 96 Plus Miniprep and BioRobot Kits allow plasmid preparation in 96-well format. The QIAprep 96 Plus Miniprep protocol is based on a modified alkaline lysis procedure. Following lysate clearing, a simple bind-wash-elute procedure results in highly concentrated plasmid DNA, ready for direct use in subsequent applications (see figure "QIAprep 96 Plus Miniprep procedure"). The unique kit chemistry and design of the Plasmid Plus 96 plates allow high binding capacities. After neutralization, a TurboFilter 96 plate is used for lysate clearing. To optimize plasmid DNA binding to the membrane of the Plasmid Plus 96 plate, a nonchaotropic binding buffer is added to the cleared lysate. The unique binding buffer ensures very specific binding conditions, resulting in DNA quality that is comparable to anion-exchange preps (see figure "Superior plasmid DNA quality"). Due to unique kit chemistry, up to 50 μg plasmid DNA per well can be obtained from a 5 ml E. coli culture (see figures "High yields of plasmid DNA", "The QIAprep 96 Plus Miniprep Kit outperforms kits from other suppliers", and "High yields of plasmid DNA with the QIAprep 96 Plus Miniprep Kit"). High-purity preps and outstanding yields QIAprep 96 Plus Miniprep and BioRobot Kits provide a novel method for preparation of highly pure plasmid DNA (see figure "Superior plasmid DNA quality"). Yields of up to 50 μg can be consistently achieved using an optimized procedure. The unique binding buffer provided with kits ensures very specific binding conditions (see figures "High yields of plasmid DNA", "The QIAprep 96 Plus Miniprep Kit outperforms kits from other suppliers", and "High yields of plasmid DNA with the QIAprep 96 Plus Miniprep Kit"). Convenient and flexible processing The QIAprep 96 Plus Miniprep Kit can be conveniently processed using the QIAvac 96 and/or a centrifuge, while preps can be automated on the BioRobot Universal System using the QIAprep 96 Plus BioRobot Kit. Optimized protocols ensure easy and straightforward plasmid DNA preparation in 96-well format.