30002 Strep Tactin Superflow Plus;Qiagen 30002;原装

产地：Qiagen货号：30002ApplicationsStrep-Tactin Superflow Plus can be used for efficient and reproducible purification of up to 9 mg Strep-tagged protein per ml resin. Purified proteins can be used for functional or interaction studies, crystallization screening, or for generation of antibodies. ImagesProtein binding capacities of Strep-Tactin matrices.Binding of Strep-tagged proteins to Strep-Tactin and elution using desthiobiotin. Efficient purification of Strep-tagged GPF with Strep-Tactin Superflow Plus. Highly specific detection of Strep-tagged proteins in any expression system.  Reagents compatible with the Strep-tag/Strep-Tactin interaction. Principle and procedureStrep-tagged proteins bind with high affinity and specificity to Strep-Tactin, an engineered form of streptavidin (see figure "Protein binding capacities of Strep-Tactin matrices "). After washing, proteins are eluted using low concentrations of biotin (the natural ligand of streptavidin) or desthiobiotin (a stable, reversibly binding biotin analog) (see Figure "Binding of Strep-tagged proteins to Strep-Tactin and elution using desthiobiotin"). Labeling proteins with the small Strep-tag II enables efficient, standardized purification of recombinant proteins expressed in prokaryotic and eukaryotic systems, and delivers highly pure proteins (see figure "Efficient purification of Strep-tagged GPF with Strep-Tactin Superflow Plus"). In addition, the Strep-Tag II is the system of choice in situations where other tags may be unsuitable, e.g., when buffer additives such as EDTA may strip IMAC columns. Strep-tagged proteins are expressed using the pQE TriSystem Strep Vector and obtained at very high purity from cell lysates using Strep-Tactin Superflow Plus affinity purification procedures. Strep-tagged proteins can be purified using batch or gravity-flow methods, or FPLC-compatible columns can be filled with Strep-Tactin Superflow Plus for purification on liquid chromatography systems. Strep-Tactin Superflow Plus is available in pre-filled 1 ml cartridges and 5 ml cartridges for automated purification on liquid chromatography systems such as the FPLC, AKTA, and BioLogic systems, or manual purification using a syringe. The C-terminal location of the tag ensures that only full-length proteins are purified. The purification process is highly reproducible, guaranteeing the same high quality protein preparations time after time. Monoclonal Strep-tag antibodies are used to detect Strep-tagged proteins in immunodetection procedures with high sensitivity and specificity (see Figure "Highly specific detection of Strep-tagged proteins in any expression system"). pQE TriSystem His•Strep Vectors can be used to express proteins doubly-tagged with a His- and a Strep-tag, which are purified in a two-step affinity procedure. Sequential Ni-NTA and Strep-Tactin purification steps are standardized, very fast, and deliver ultrapure proteins.