商品分类：单克隆抗体
产品概述：Vimentin is the most common member of intermediate filament (IF) family and one of the main components in cytoskeleton structure. It is expressed in during cell development and differentiation in variety of mescencymal cells and cell types derived from mesoderm (1). Vimentin is essential in the role of cell integrity and cytoskeletal stability. The reorganization of Vimentin, similar to all IF proteins, occurs during different stages of the cell cycle and cell signaling by a site-specific phosphorylation (serine and threonine residues) (2). In particular, p21-activated kinase (PAK) phosphorylates at Ser25, Ser38, Ser50, Ser65 and Ser72 which induces Vimentin specific reorganization (3). During cytokinesis, Vimentin is regulated by Rho-kinase (ROCK) and Aurora B through phosphorylation at Ser38 and Ser72 (4). Also, coordinated by ROCK and Aurora B, Plk1 induces phosphorylation at Ser82 plays an important role in Vimentin segregation (5).
靶点/蛋白：A synthetic acetylated peptide corresponding to residues surrounding Ser82 of Vimentin was used as an immunogen. The antibody only detects Vimentin phosphorylated at Serine 82.
来源：Rabbit
存储溶液：50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
参考文献：1. Eriksson JE et al. Curr. Opin. Cell Biol. 4:99-104, 1992.
2. Inagaki M et al. Nature 328: 649-652, 1987.
3. Goto H et al. GENES CELLS 7:91-97. 2002.
4. Goto H et al. J Biol Chem, 273(19):11728-11736, 1998.
5. Yamaguchi T et al. JCB, 171(3):431-436, 2005.
特异性：Human, Mouse, Rat
别名：Vimentin, VIM
应用：WB~~1:10000~50000
简单描述：Rabbit Monoclonal Vimentin Antibody Phospho (pS82). Validated for use in WB. Clone: EP1071Y.
详情：FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.Cellular_LocationCytoplasm.Tissue_LocationHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
生物商城：http://www.annoron.com
办公电话：010-56256916
官方售后：400-965-8633
企业 QQ：3212874516、3198592576
地 址：北京大兴经济技术开发区(亦庄)同济中路7号兴盛国际A栋4层
温馨提示：不可用于临床ZL。
