Mouse monoclonal antibody
HYB 094-07 reacts with denatured ovalbumin. Cross-reactivity with ovalbumins from other avian species has not been tested.
Purified chicken ovalbumin was treated with formaldehyde and lysine for 2 weeks at 35°C and then autoclaved for 1 h at 110°C
IgG2a/k
Epitope specificity differs from that of HYB 094-05 and HYB 094-06, as indicated by lack of mutual inhibition of antigen binding.
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Ovalbumin is the main protein found in egg white, making up 60-65% of the total protein. Native chicken ovalbumin is a globular monomer with a molecular mass of 45 kDa. It is structurally related to both serpins and lipocalins.
ELISA
HYB 094-07 reacts with chicken ovalbumin in denatured or modified forms (surface-adsorbed, heat-denatured or reduced), including native chicken ovalbumin coated directly onto the microtiter well.
WB
In Western blotting after SDS-PAGE, HYB 094-07 reacts with ovalbumin in both reduced and unreduced forms. A dilution guideline of 1/1000 has proved successful.
