pMT-Bip-V5-HisA

pMT-Bip-V5-HisA载体基本信息

出品公司:	Ybscience
载体名称:	pMT-Bip-V5-HisA
质粒类型:	昆虫细胞表达载体
高拷贝/低拷贝:	--
启动子:	P-MT (Metallothionein)
克隆方法:	多克隆位点，限制性内切酶
载体大小:	3642bp
5' 测序引物及序列:	Metallothionein: 5'd[CATCTCAGTGCAACTAAA]3'
3' 测序引物及序列:	--
载体标签:	BiP (N), V5 (C), His (C)
载体抗性:	Ampicillin
筛选标记:	--
备注:	使用硫酸铜或氯化镉作为诱导剂。
稳定性:	--
组成型:	诱导型
病毒/非病毒:	非病毒

 载体质粒图谱和多克隆位点信息

载体简介

 

 

载体序列

LOCUS       pMT/BIP/V5-HisA           3642 bp ds-DNA    circular SYN 09-四月-2014DEFINITION  Drosophila vector for inducible high-level expression of secreted             and tagged proteins. For other reading frames, use pMT/BiP/V5-His B             or pMT/BiP/V5-His C.ACCESSION   .VERSION     .KEYWORDS    pMT BiP V5-His ASOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructCOMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     VNTAUTHORNAME|biofeng.com|COMMENT     To create stable cell lines, co-transfect with the drug selection             vector pCoHygro or pCoBlast.FEATURES             Location/Qualifiers     source          1..3642                     /organism="synthetic DNA construct"                     /lab_host="Insect Cells"                     /mol_type="other DNA"     promoter        411..833                     /label="MT promoter"                     /label="Drosophila metallothionein promoter"     CDS             851..904                     /codon_start=1                     /product="Drosophila BiP signal sequence"                     /label="BiP signal sequence"                     /translation="MKLCILLAVVAFVGLSLG"     misc_feature    905..1002                     /label="MCS"                     /label="multiple cloning site"     CDS             1003..1044                     /codon_start=1                     /product="epitope tag from simian virus 5"                     /label="V5 tag"                     /translation="GKPIPNPLLGLDST"     CDS             1054..1071                     /codon_start=1                     /product="6xHis affinity tag"                     /label="6xHis"                     /translation="HHHHHH"     polyA_signal    1144..1278                     /label="SV40 poly(A) signal"                     /label="SV40 polyadenylation signal"     rep_origin      complement(1824..2412)                     /direction=LEFT                     /label="ori"                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"     CDS             complement(2583..3443)                     /codon_start=1                     /gene="bla"                     /product="beta-lactamase"                     /label="AmpR"                     /label="confers resistance to ampicillin, carbenicillin, and                     related antibiotics"     promoter        complement(3444..3548)                     /gene="bla"                     /label="AmpR promoter"ORIGIN        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccag tgaattttaa cgttgcagga      421 caggatgtgg tgcccgatgt gactagctct ttgctgcagg ccgtcctatc ctctggttcc      481 gataagagac ccagaactcc ggccccccac cgcccaccgc cacccccata catatgtggt      541 acgcaagtaa gagtgcctgc gcatgcccca tgtgccccac caagagtttt gcatcccata      601 caagtcccca aagtggagaa ccgaaccaat tcttcgcggg cagaacaaaa gcttctgcac      661 acgtctccac tcgaatttgg agccggccgg cgtgtgcaaa agaggtgaat cgaacgaaag      721 acccgtgtgt aaagccgcgt ttccaaaatg tataaaaccg agagcatctg gccaatgtgc      781 atcagttgtg gtcagcagca aaatcaagtg aatcatctca gtgcaactaa aggggggatc      841 cgatctcaat atgaagttat gcatattact ggccgtcgtg gcctttgttg gcctctcgct      901 cgggagatct ccatggcccg gggtacctac tagtccagtg tggtggaatt ctgcagatat      961 ccagcacagt ggcggccgct cgagtctaga gggcccttcg aaggtaagcc tatccctaac     1021 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa     1081 acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc     1141 gaggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag     1201 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata     1261 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg     1321 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat     1381 gatcagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg     1441 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa     1501 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct     1561 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga     1621 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc     1681 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     1741 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt     1801 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa     1861 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt     1921 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg     1981 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc     2041 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc     2101 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta     2161 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct     2221 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc     2281 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa     2341 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa     2401 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa     2461 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt     2521 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac     2581 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc     2641 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc     2701 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata     2761 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc     2821 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc     2881 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca     2941 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa     3001 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca     3061 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt     3121 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt     3181 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg     3241 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga     3301 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc     3361 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg     3421 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag     3481 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     3541 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg     3601 acattaacct ataaaaatag gcgtatcacg aggccctttc gt