Anti-Glutathione-S-transferase (GST) monoclonal antibody

Background
Glutathione-S-Transferase (GST) is an enzyme, which catalyzes the reaction of glutathione with a wide range of electrophilic substrates and therefore plays a role in the detoxification of potential alkylating agents. GST from Schistosoma japonicum, has been cloned in several expression vectors as a 27.5 kDa molecule, and used as a tag for expressing proteins of interest. These proteins are thus expressed as GST-fusion proteins. Antibodies to GST are useful in identifying the successful expression of these fusion proteins.
Description
a mouse monoclonal antibody raised against Glutathione-S-Transferase.
Source
Glutathione-S-Transferase.
Applications
WB 1:1000-2000, ELISA 1:500-2000. Optimal dilution has to be determined by the user.
Applicable Species
GST from Schistosoma japonicum in GST fusion proteins.
Specifications
Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4, 0.5% BSA with 0.05% sodium azide. Antibody was purified by affinity chromatography.
Storage conditions
store at 2-8℃ for 3 months, -20℃ for 1 year. To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20℃) or for long-term storage (-80℃).
Note
For research use only, not for use in diagnostic procedures.
Data


Western blot analysis of GST-tagged fusion proteins.