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载体pBI221-EGFP

产品信息
  • pBI221-EGFP
    产品信息
    产品货号 产品名称 产品规格 优惠价
    HZ0287 pBI221-EGFP

    20μl

    ¥请询价
    使用说明
    沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。
    基本信息
    启动子: CaMV 35S ,Lac
    复制子: ori
    终止子: NOS
    质粒分类: 植物系列,蛋白过表达载体
    质粒大小: 4530bp
    原核抗性: Amp
    克隆菌株: DH5α
    培养条件: 37℃,有氧 LB
    5'测序引物: 35S:GACGCACAATCCCACTATCC
    3'测序引物: 根据序列设计引物
    质粒简介
         Most studies related to determining the expression profile of genes and specific promoters used histochemical localization of the reporter gene, gusA. While the histochemical method for visualizing gusA expression suffers from several limitations in the determination of gene expression and location, especially in the tissues with high background acitivty. To solve this problem, a transient expession vector pBI221-RFP/GFP, was constructed using GFP and RFP as double fluorescent marker genes. This vector used CaMV 35S promoter to drive GFP and determine the transforming efficiency. It analyzed expression profile of the target gene and promoter through the RFP activities of the tranformed tissues. Through the specific promoter AGPL1 from watermelon and E8 promoter from tomato, it is resistible to use this vector to study the expression patterns of promoters. Results indicated that the pBI221-RFP/GFP is a very efficient transient expression vector that can be verify the functions of the genes and promoters. 
    质粒图谱
    温馨提示:不可用于临床ZL。
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