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载体pLVX-EF1α-IRES-puro

产品信息
  • pLVX-EF1α-IRES-puro
    产品信息
    产品货号 产品名称 产品规格 优惠价
    HZ0405 pLVX-EF1α-IRES-puro

    20μl

    ¥请询价
    使用说明
    沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。
    基本信息
    启动子: EF-1α
    复制子: pUC ori
    终止子: SV40 poly(A) signal
    质粒分类: 病毒系列,慢病毒克隆载体
    质粒大小: 8825bp
    原核抗性: Amp
    筛选标记: Puro
    克隆菌株: Stbl3
    培养条件: 37℃,有氧 LB
    表达宿主: 哺乳细胞
    诱导方式: 无须诱导,瞬时表达
    5'测序引物: 根据序列设计引物
    3'测序引物: 根据序列设计引物
    质粒简介
        pLVX-EF1α-IRES-Puro contains all of the viral processing elements necessary for the production of replicationincompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral RNA, leading to increased viral titers from packaging cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus. Finally, pLVX-EF1α-IRES-Puro also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, this element creates a central DNA flap that increases nuclear import ofthe viral genome, resulting in improved vector integration and more efficient transduction. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.
    质粒图谱
    温馨提示:不可用于临床ZL。
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