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载体pCL-Eco

产品信息
  • pCL-Eco
    产品信息
    产品货号 产品名称 产品规格 优惠价
    HZ0334 pCL-Eco

    20μl

    ¥请询价
    使用说明
    沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。
    基本信息
    启动子: CMV
    复制子: pUC ori,SV40 ori
    终止子: SV40 poly(A) signal
    质粒分类: 病毒系列,逆转录病毒包装载体图
    质粒大小: 12341bp
    质粒标签: gag-pol
    原核抗性: Amp
    克隆菌株: Stbl3
    培养条件: 37℃,有氧 LB
    表达宿主: 哺乳细胞
    诱导方式: 无须诱导,瞬时表达
    5'测序引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
    3'测序引物: 根据序列设计引物
    质粒简介
        The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of about 1 infectious particle/cell/day. Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. gp70 comes in three different flavors for gene therapy. Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells.
    质粒图谱
    温馨提示:不可用于临床ZL。
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