pShuttle-IRES-hrGFP-2载体
- 品牌：XYbscience
- 型号：信裕生物
- 产地：中国/美国
- 供应商：上海信裕生物科技有限公司
- 供应商报价：¥1500
- 标签：pShuttle-IRES-hrGFP-2载体、pShuttle-IRES-hrGFP-2载体价格、pShuttle-IRES-hrGFP-2载体厂家、pShuttle-IRES-hrGFP-2、、上海信裕生物科技有限公司
产品库
| 质粒类型: | 腺病毒系统 |
|---|---|
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8.9 kb |
| 5' 测序引物及序列: | pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC |
| 3' 测序引物及序列: | pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG |
| 载体抗性: | Kanamycin (卡那霉素) |
| 备注: | 载体内包含有IRES序列和hrGFP序列 |
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| XY1496 | pShuttle-IRES-hrGFP-2 | 5ug质粒 | ¥1500.00 |
Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG® epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).
